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dc.contributor.authorEpler, Chelsea R.
dc.contributor.authorDickenson, Nicholas E.
dc.contributor.authorOlive, Andrew J.
dc.contributor.authorPicking, Wendy Lynn
dc.contributor.authorPicking, William D.
dc.date.accessioned2016-08-15T16:59:34Z
dc.date.available2016-08-15T16:59:34Z
dc.date.issued2009-07
dc.identifier.citationEpler, C. R., Dickenson, N. E., Olive, A. J., Picking, W. L., & Picking, W. D. (2009). Liposomes Recruit IpaC to the Shigella flexneri Type III Secretion Apparatus Needle as a Final Step in Secretion Induction . Infection and Immunity, 77(7), 2754–2761. http://doi.org/10.1128/IAI.00190-09en_US
dc.identifier.urihttp://hdl.handle.net/1808/21324
dc.description.abstractShigella flexneri contact with enterocytes induces a burst of protein secretion via its type III secretion apparatus (TTSA) as an initial step in cellular invasion. We have previously reported that IpaD is positioned at the TTSA needle tip (M. Espina et al., Infect. Immuno. 74:4391-4400, 2006). From this position, IpaD senses small molecules in the environment to control the presentation of IpaB to the needle tip. This step occurs without type III secretion induction or IpaC recruitment to the S. flexneri surface. IpaC is then transported to the S. flexneri surface when target cell lipids are added, and this event presumably mimics host cell contact. Unlike IpaB mobilization, IpaC surface presentation is closely linked to secretion induction. This study demonstrates that sphingomyelin and cholesterol are key players in type III secretion induction and that they appear to interact with IpaB to elicit IpaC presentation at the TTSA needle tip. Furthermore, IpaB localization at the needle tip prior to membrane contact provides the optimal set of conditions for host cell invasion. Thus, the S. flexneri type III secretion system can be induced in a stepwise manner, with the first step being the stable association of IpaD with the needle tip, the second step being the sensing of small molecules by IpaD to mobilize IpaB to the tip, and the third step being the interaction of lipids with IpaB to induce IpaC localization at the needle tip concomitant with translocon insertion into the host membrane and type III secretion induction.en_US
dc.publisherAmerican Society for Microbiologyen_US
dc.rightsCopyright © 2009, American Society for Microbiologyen_US
dc.titleLiposomes Recruit IpaC to the Shigella flexneri Type III Secretion Apparatus Needle as a Final Step in Secretion Induction0en_US
dc.typeArticleen_US
kusw.kuauthorPicking, Wendy Lynn
kusw.kuauthorPicking, William D.
kusw.kudepartmentPharmaceutical Chemistryen_US
dc.identifier.doi10.1128/IAI.00190-09en_US
dc.identifier.orcidhttps://orcid.org/0000-0003-1572-6077 https://orcid.org/0000-0003-3441-3113 https://orcid.org/0000-0001-7998-0643
kusw.oaversionScholarly/refereed, publisher versionen_US
kusw.oapolicyThis item meets KU Open Access policy criteria.en_US
dc.rights.accessrightsopenAccess


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