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Endotoxicosis Induced by Coxiella burnetii Lipopolysaccharide Stimulates a Ribosomal Protein S6 Kinase: Some Properties of the Partially Purified Enzyme
dc.contributor.author | Picking, William D. | |
dc.contributor.author | Hackstadt, T. | |
dc.contributor.author | Paretsky, D. | |
dc.date.accessioned | 2016-08-15T16:35:36Z | |
dc.date.available | 2016-08-15T16:35:36Z | |
dc.date.issued | 1989-12 | |
dc.identifier.citation | Picking, W. D., Hackstadt, T., & Paretsky, D. (1989). Endotoxicosis induced by Coxiella burnetii lipopolysaccharide stimulates a ribosomal protein S6 kinase: some properties of the partially purified enzyme. Infection and Immunity, 57(12), 3683–3688. | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/21321 | |
dc.description.abstract | Guinea pig endotoxicosis induced by lipopolysaccharide from Coxiella burnetii Nine Mile phase I stimulates phosphorylation of liver ribosomal protein S6, with a 50% increase at 12 h postinoculation. The responsible protein kinase (S6PK) has been partially purified from liver; its activity is independent of cyclic AMP and of Ca2+ plus phosphatidyl serine or diacylglycerol. The preparation has an apparent optimum concentration of 20 mM Mg2+, while Ca2+ and Mn2+ are each inhibitory at 2 mM. The apparent Km for ATP is 30 microM with intact ribosomes. Because of the central role of phosphorylation in metabolic regulation and a purported role of phosphorylated S6 in protein synthesis, the lipopolysaccharide-induced stimulation of S6PK suggests a significant regulatory role of such enzymes in the pathobiochemistry of Q fever infection and endotoxicosis. | en_US |
dc.publisher | American Society for Microbiology | en_US |
dc.relation.isversionof | http://iai.asm.org/content/57/12/3683.long | en_US |
dc.title | Endotoxicosis Induced by Coxiella burnetii Lipopolysaccharide Stimulates a Ribosomal Protein S6 Kinase: Some Properties of the Partially Purified Enzyme | en_US |
dc.type | Article | en_US |
kusw.kuauthor | Picking, William D. | |
kusw.kudepartment | Pharmaceutical Chemistry | en_US |
kusw.oaversion | Scholarly/refereed, publisher version | en_US |
kusw.oapolicy | This item does not meet KU Open Access policy criteria. | en_US |
dc.rights.accessrights | openAccess |