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dc.contributor.authorOaks, Edwin V.
dc.contributor.authorPicking, William D.
dc.contributor.authorPicking, Wendy Lynn
dc.date.accessioned2016-08-15T16:09:07Z
dc.date.available2016-08-15T16:09:07Z
dc.date.issued1996-01-18
dc.identifier.citationOaks, E. V., Picking, W. D., & Picking, W. L. (1996). Antibody response of monkeys to invasion plasmid antigen D after infection with Shigella spp. Clinical and Diagnostic Laboratory Immunology, 3(2), 242–245.en_US
dc.identifier.urihttp://hdl.handle.net/1808/21318
dc.description.abstractThe antigen preparation most often used for determining the levels of antibodies to virulence-associated proteins of Shigella spp. consists of a mixture of proteins (including IpaB, IpaC, IpaD, and VirG*) extracted from virulent shigellae with water (water extract). To overcome the lack of specificity for individual antigens in the water-extract enzyme-linked immunosorbent assay (ELISA), the ipaD gene from S. flexneri has been cloned, expressed to a high level, and purified for use in a new ELISA for the determination of the levels of antibody against IpaD in monkeys and humans challenged with shigellae. The IpaD ELISA for serum immunoglobulins G and A correlated well with the water-extract ELISA in that monkeys infected with S. flexneri or S. sonnei responded with high serum antibody titers in both assays. The IpaD assay required less antigen per well, had much lower background levels, and did not require correction with antigens from an avirulent organism. In conjunction with the water-extract ELISA, it was possible to identify infected animals that did not respond to IpaD but did produce antibodies that reacted in the water-extract ELISA. This indicates that even though IpaB, IpaC, and IpaD are essential for the invasiveness phenotype, the infected host does not always produce antibodies against all components of the invasiveness apparatus.en_US
dc.publisherAmerican Society for Microbiologyen_US
dc.relation.isversionofhttp://cvi.asm.org/content/3/2/242.longen_US
dc.titleAntibody Response of Monkeys to Invasion Plasmid Antigen D after Infection with Shigella spp.en_US
dc.typeArticleen_US
kusw.kuauthorPicking, William D.
kusw.kuauthorPicking, Wendy Lynn
kusw.kudepartmentPharmaceutical Chemistryen_US
dc.identifier.orcidhttps://orcid.org/0000-0001-7998-0643
kusw.oaversionScholarly/refereed, publisher versionen_US
kusw.oapolicyThis item does not meet KU Open Access policy criteria.en_US
dc.rights.accessrightsopenAccess


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