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dc.contributor.authorDeane, Janet E.
dc.contributor.authorCordes, Frank S.
dc.contributor.authorRoversi, Pietro
dc.contributor.authorJohnson, Steven
dc.contributor.authorKenjale, Roma
dc.contributor.authorPicking, William D.
dc.contributor.authorPicking, Wendy Lynn
dc.contributor.authorLea, Susan M.
dc.contributor.authorBlocker, Ariel J.
dc.date.accessioned2016-07-21T18:16:22Z
dc.date.available2016-07-21T18:16:22Z
dc.date.issued2006-02-28
dc.identifier.citationDeane, J. E., Cordes, F. S., Roversi, P., Johnson, S., Kenjale, R., Picking, W. D., … Blocker, A. (2006). Expression, purification, crystallization and preliminary crystallographic analysis of MxiH, a subunit of the Shigella flexneri type III secretion system needle. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 62(Pt 3), 302–305. http://doi.org/10.1107/S1744309106006555en_US
dc.identifier.urihttp://hdl.handle.net/1808/21170
dc.description.abstractA monodisperse truncation mutant of MxiH, the subunit of the needle from the Shigella flexneri type III secretion system (TTSS), has been overexpressed and purified. Crystals were grown of native and selenomethionine-labelled MxiHCΔ5 and diffraction data were collected to 1.9 Å resolution. The crystals belong to space group C2, with unit-cell parameters a = 183.4, b = 28.1, c = 27.8 Å, β = 96.5°. An anomalous difference Patterson map calculated with the data from the SeMet-labelled crystals revealed a single peak on the Harker section v = 0. Inspection of a uranyl derivative also revealed one peak in the isomorphous difference Patterson map on the Harker section v = 0. Analysis of the self-rotation function indicates the presence of a twofold non-crystallographic symmetry axis approximately along a. The calculated Matthews coefficient is 1.9 Å3 Da−1 for two molecules per asymmetric unit, corresponding to a solvent content of 33%.en_US
dc.publisherInternational Union of Crystallographyen_US
dc.rightsCopyright © International Union of Crystallography 2006. This is an open-access article distributed under the terms described at http://journals.iucr.org/services/termsofuse.html.en_US
dc.subjectMxiHen_US
dc.subjectType III secretion systemen_US
dc.subjectShigella flexnerien_US
dc.titleExpression, purification, crystallization and preliminary crystallographic analysis of MxiH, a subunit of the Shigella flexneri type III secretion system needleen_US
dc.title.alternativeExpression, purification, crystallization and preliminary crystallographic analysis of MxiH, a subunit of the Shigella flexneri type III secretion system needleen_US
dc.typeArticleen_US
kusw.kuauthorKenjale, Roma
kusw.kuauthorPicking, William D.
kusw.kuauthorPicking, Wendy Lynn
kusw.kudepartmentPharmaceutical Chemistryen_US
kusw.kudepartmentMolecular Biosciencesen_US
dc.identifier.doi10.1107/S1744309106006555en_US
dc.identifier.orcidhttps://orcid.org/0000-0001-7998-0643
kusw.oaversionScholarly/refereed, publisher versionen_US
kusw.oapolicyThis item does not meet KU Open Access policy criteria.en_US
dc.rights.accessrightsopenAccess


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