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dc.contributor.authorZhao, Zheng
dc.contributor.authorYang, Yang
dc.contributor.authorZeng, Yong
dc.contributor.authorHe, Mei
dc.date.accessioned2016-04-14T19:33:21Z
dc.date.available2016-04-14T19:33:21Z
dc.date.issued2015-11-20
dc.identifier.citationZeng, Yong, Mei He et al. (2016) A microfluidic ExoSearch chip for multiplexed exosome detection towards blood-based ovarian cancer diagnosis. Lab on a Chip, 16, 489. DOI:10.1039/c5lc01117een_US
dc.identifier.urihttp://hdl.handle.net/1808/20676
dc.description.abstractTumor-derived circulating exosomes, enriched with a group of tumor antigens, have been recognized as a promising biomarker source for cancer diagnosis via a less invasive procedure. Quantitatively pinpointing exosome tumor markers is appealing, yet challenging. In this study, we developed a simple microfluidic approach (ExoSearch) which provides enriched preparation of blood plasma exosomes for in situ, multiplexed detection using immunomagnetic beads. The ExoSearch chip offers a robust, continuous-flow design for quantitative isolation and release of blood plasma exosomes in a wide range of preparation volumes (10 μL to 10 mL). We employed the ExoSearch chip for blood-based diagnosis of ovarian cancer by multiplexed measurement of three exosomal tumor markers (CA-125, EpCAM, CD24) using a training set of ovarian cancer patient plasma, which showed significant diagnostic power (a.u.c. = 1.0, p = 0.001) and was comparable with the standard Bradford assay. This work provides an essentially needed platform for utilization of exosomes in clinical cancer diagnosis, as well as fundamental exosome research.en_US
dc.publisherRSC Publishingen_US
dc.rightsThis article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence.en_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc/3.0/
dc.titleA microfluidic ExoSearch chip for multiplexed exosome detection towards blood-based ovarian cancer diagnosisen_US
dc.typeArticleen_US
kusw.kuauthorZeng, Yong
kusw.kudepartmentChemistryen_US
dc.identifier.doi10.1039/c5lc01117een_US
dc.identifier.orcidhttps://orcid.org/0000-0001-7068-1321
kusw.oaversionScholarly/refereed, publisher versionen_US
kusw.oapolicyThis item meets KU Open Access policy criteria.en_US
dc.rights.accessrightsopenAccess


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