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A microfluidic ExoSearch chip for multiplexed exosome detection towards blood-based ovarian cancer diagnosis
dc.contributor.author | Zhao, Zheng | |
dc.contributor.author | Yang, Yang | |
dc.contributor.author | Zeng, Yong | |
dc.contributor.author | He, Mei | |
dc.date.accessioned | 2016-04-14T19:33:21Z | |
dc.date.available | 2016-04-14T19:33:21Z | |
dc.date.issued | 2015-11-20 | |
dc.identifier.citation | Zeng, Yong, Mei He et al. (2016) A microfluidic ExoSearch chip for multiplexed exosome detection towards blood-based ovarian cancer diagnosis. Lab on a Chip, 16, 489. DOI:10.1039/c5lc01117e | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/20676 | |
dc.description.abstract | Tumor-derived circulating exosomes, enriched with a group of tumor antigens, have been recognized as a promising biomarker source for cancer diagnosis via a less invasive procedure. Quantitatively pinpointing exosome tumor markers is appealing, yet challenging. In this study, we developed a simple microfluidic approach (ExoSearch) which provides enriched preparation of blood plasma exosomes for in situ, multiplexed detection using immunomagnetic beads. The ExoSearch chip offers a robust, continuous-flow design for quantitative isolation and release of blood plasma exosomes in a wide range of preparation volumes (10 μL to 10 mL). We employed the ExoSearch chip for blood-based diagnosis of ovarian cancer by multiplexed measurement of three exosomal tumor markers (CA-125, EpCAM, CD24) using a training set of ovarian cancer patient plasma, which showed significant diagnostic power (a.u.c. = 1.0, p = 0.001) and was comparable with the standard Bradford assay. This work provides an essentially needed platform for utilization of exosomes in clinical cancer diagnosis, as well as fundamental exosome research. | en_US |
dc.publisher | RSC Publishing | en_US |
dc.rights | This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by-nc/3.0/ | |
dc.title | A microfluidic ExoSearch chip for multiplexed exosome detection towards blood-based ovarian cancer diagnosis | en_US |
dc.type | Article | en_US |
kusw.kuauthor | Zeng, Yong | |
kusw.kudepartment | Chemistry | en_US |
dc.identifier.doi | 10.1039/c5lc01117e | en_US |
dc.identifier.orcid | https://orcid.org/0000-0001-7068-1321 | |
kusw.oaversion | Scholarly/refereed, publisher version | en_US |
kusw.oapolicy | This item meets KU Open Access policy criteria. | en_US |
dc.rights.accessrights | openAccess |