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Stem Cells in Aggregate Form to Enhance Chondrogenesis in Hydrogels
dc.contributor.author | Sridharan, BanuPriya | |
dc.contributor.author | Lin, Staphany M. | |
dc.contributor.author | Hwu, Alexander T. | |
dc.contributor.author | Laflin, Amy D. | |
dc.contributor.author | Detamore, Michael S. | |
dc.date.accessioned | 2016-03-29T15:33:26Z | |
dc.date.available | 2016-03-29T15:33:26Z | |
dc.date.issued | 2015-12-31 | |
dc.identifier.citation | Sridharan B, Lin SM, Hwu AT, Laflin AD, Detamore MS (2015) Stem Cells in Aggregate Form to Enhance Chondrogenesis in Hydrogels. PLoS ONE 10(12): e0141479. doi:10.1371/journal.pone.0141479 | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/20581 | |
dc.description.abstract | There are a variety of exciting hydrogel technologies being explored for cartilage regenerative medicine. Our overall goal is to explore whether using stem cells in an aggregate form may be advantageous in these applications. 3D stem cell aggregates hold great promise as they may recapitulate the in vivo skeletal tissue condensation, a property that is not typically observed in 2D culture. We considered two different stem cell sources, human umbilical cord Wharton’s jelly cells (hWJCs, currently being used in clinical trials) and rat bone marrow-derived mesenchymal stem cells (rBMSCs). The objective of the current study was to compare the influence of cell phenotype, aggregate size, and aggregate number on chondrogenic differentiation in a generic hydrogel (agarose) platform. Despite being differing cell sources, both rBMSC and hWJC aggregates were consistent in outperforming cell suspension control groups in biosynthesis and chondrogenesis. Higher cell density impacted biosynthesis favorably, and the number of aggregates positively influenced chondrogenesis. Therefore, we recommend that investigators employing hydrogels consider using cells in an aggregate form for enhanced chondrogenic performance. | en_US |
dc.publisher | Public Library of Science | en_US |
dc.rights | © 2015 Sridharan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.title | Stem Cells in Aggregate Form to Enhance Chondrogenesis in Hydrogels | en_US |
dc.type | Article | en_US |
kusw.kuauthor | Sridharan, BanuPriya | |
kusw.kuauthor | Lin, Staphany M. | |
kusw.kuauthor | Hwu, Alexander T. | |
kusw.kuauthor | Laflin, Amy D. | |
kusw.kuauthor | Detamore, Michael S. | |
kusw.kudepartment | Chemical and Petroleum Engineering | en_US |
kusw.oastatus | fullparticipation | en_US |
dc.identifier.doi | 10.1371/journal.pone.0141479 | en_US |
kusw.oaversion | Scholarly/refereed, author accepted manuscript | en_US |
kusw.oapolicy | This item meets KU Open Access policy criteria. | en_US |
dc.rights.accessrights | openAccess |
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Except where otherwise noted, this item's license is described as: © 2015 Sridharan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.