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Integrated Microfluidic Lectin Barcode Platform for High-Performance Focused Glycomic Profiling
dc.contributor.author | Shang, Yuqin | |
dc.contributor.author | Zeng, Yun | |
dc.contributor.author | Zeng, Yong | |
dc.date.accessioned | 2016-03-07T21:20:03Z | |
dc.date.available | 2016-03-07T21:20:03Z | |
dc.date.issued | 2016-02-02 | |
dc.identifier.citation | Integrated Microfluidic Lectin Barcode Platform for High-Performance Focused Glycomic Profiling Yuqin Shang, Yun Zeng, Yong Zeng Sci Rep. 2016; 6: 20297. Published online 2016 February 2. doi: 10.1038/srep20297 | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/20483 | |
dc.description | A grant from the One-University Open Access Fund at the University of Kansas was used to defray the author's publication fees in this Open Access journal. The Open Access Fund, administered by librarians from the KU, KU Law, and KUMC libraries, is made possible by contributions from the offices of KU Provost, KU Vice Chancellor for Research & Graduate Studies, and KUMC Vice Chancellor for Research. For more information about the Open Access Fund, please see http://library.kumc.edu/authors-fund.xml. | |
dc.description.abstract | Protein glycosylation is one of the key processes that play essential roles in biological functions and dysfunctions. However, progress in glycomics has considerably lagged behind genomics and proteomics, due in part to the enormous challenges in analysis of glycans. Here we present a new integrated and automated microfluidic lectin barcode platform to substantially improve the performance of lectin array for focused glycomic profiling. The chip design and flow control were optimized to promote the lectin-glycan binding kinetics and speed of lectin microarray. Moreover, we established an on-chip lectin assay which employs a very simple blocking method to effectively suppress the undesired background due to lectin binding of antibodies. Using this technology, we demonstrated focused differential profiling of tissue-specific glycosylation changes of a biomarker, CA125 protein purified from ovarian cancer cell line and different tissues from ovarian cancer patients in a fast, reproducible, and high-throughput fashion. Highly sensitive CA125 detection was also demonstrated with a detection limit much lower than the clinical cutoff value for cancer diagnosis. This microfluidic platform holds the potential to integrate with sample preparation functions to construct a fully integrated “sample-to-answer” microsystem for focused differential glycomic analysis. Thus, our technology should present a powerful tool in support of rapid advance in glycobiology and glyco-biomarker development. | en_US |
dc.publisher | Nature Publishing Group | en_US |
dc.rights | This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.title | Integrated Microfluidic Lectin Barcode Platform for High-Performance Focused Glycomic Profiling | en_US |
dc.type | Article | |
kusw.kuauthor | Shang, Yuqin | |
kusw.kudepartment | Chemistry | en_US |
dc.identifier.doi | 10.1038/srep20297 | |
kusw.oaversion | Scholarly/refereed, publisher version | |
kusw.oapolicy | This item meets KU Open Access policy criteria. | |
dc.rights.accessrights | openAccess |
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Except where otherwise noted, this item's license is described as: This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/