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    Direct bioelectrocatalysis at the interfaces by genetically engineered dehydrogenase

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    tamerler_interfaces.pdf (2.094Mb)
    Issue Date
    2014-12-13
    Author
    Yucesoy, Deniz Tanil
    Karaca, Banu Taktak
    Cetinel, Sibel
    Caliskan, Huseyin Burak
    Adali, Esref
    Gul-Karaguler, Nevin
    Tamerler, Candan
    Publisher
    Thomas Telford
    Type
    Article
    Article Version
    Scholarly/refereed, publisher version
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    Abstract
    There is an emerging interest in developing bio-functionalisation routes serving as platforms for assembling diverse enzymes onto material surfaces. Specifically, the fabrication of next-generation, laboratory-on-a-chip-based sensing and energy harvesting systems requires controlled orientation and organisation of the proteins at the inorganic interfaces. Herein, the authors take the initial steps towards designing multifunctional, enzyme-based platforms by genetically integrating the engineered materialselective peptide tags for tethering redox enzymes onto electrode surfaces. The authors engineered a fusion protein that genetically conjugates gold-binding peptide to formate dehydrogenase derived from Candida methylica. The expressed proteins were tested for both enzyme activity and self-directed gold-surface functionalisation ability. Their findings demonstrate the successful self-immobilisation of the engineered enzyme onto different gold electrodes while retaining the catalytic activity. Building on the functionalisation by the peptides, a fusion enzyme-integrated circuit-based biosensor system was designed. The catalytic conversion of the formate by the engineered dehydrogenase was successfully monitored on the electrode surface at subsequent intervals. The engineered peptide-mediated self-integrated electrode systems can be extended to develop a wide range of biosensing and energy-harvesting platforms using different combinations of materials and biomolecules. This paper contains supporting information that will be made available online once the issue is published. In the meantime, if you wish to get a copy of the supplementary file, please contact the Journals Editor, Sarah Brown, at sarah.brown@icepublishing.com.
    Description
    This is the published version.
    URI
    http://hdl.handle.net/1808/19354
    DOI
    https://doi.org/10.1680/bbn.14.00022
    Collections
    • Bioengineering Scholarly Works [127]
    • Mechanical Engineering Scholarly Works [114]
    Citation
    Yucesoy, Deniz Tanil, Banu Taktak Karaca, Sibel Cetinel, Huseyin Burak Caliskan, Esref Adali, Nevin Gul-Karaguler, and Candan Tamerler. "Direct Bioelectrocatalysis at the Interfaces by Genetically Engineered Dehydrogenase." Bioinspired, Biomimetic and Nanobiomaterials 4.1 (2015): 79-89. http://dx.doi.org/10.1680/bbn.14.00022.

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    KU Libraries
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    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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