dc.contributor.author | Gerdts, Cory. J | |
dc.contributor.author | Elliot, Mark | |
dc.contributor.author | Lovell, Scott | |
dc.contributor.author | Mixon, Mark B. | |
dc.contributor.author | Napuli, Alberto J. | |
dc.contributor.author | Staker, Bart L. | |
dc.contributor.author | Nollert, Peter | |
dc.contributor.author | Stewart, Lance | |
dc.date.accessioned | 2015-11-20T23:02:37Z | |
dc.date.available | 2015-11-20T23:02:37Z | |
dc.date.issued | 2008 | |
dc.identifier.citation | Gerdts, Cory J., Mark Elliott, Scott Lovell, Mark B. Mixon, Alberto J. Napuli, Bart L. Staker, Peter Nollert, and Lance Stewart. "The Plug-based Nanovolume Microcapillary Protein Crystallization System (MPCS)." Acta Crystallogr D Biol Cryst Acta Cryst D Acta Crystallogr Sect D Biol Crystallogr Acta Crystallogr Sect D Acta Crystallogr D Biol Crystallogr Acta Crystallographica Section D Biological Crystallography Acta Cryst Sect D Acta Crystallogr D 64.11 (2008): 1116-122. http//dx.doi.org/10.1107/S0907444908028060 | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/18969 | |
dc.description | This is the published version. Copyright International Union of Crystallography | en_US |
dc.description.abstract | The Microcapillary Protein Crystallization System (MPCS) embodies a new semi-automated plug-based crystallization technology which enables nanolitre-volume screening of crystallization conditions in a plasticware format that allows crystals to be easily removed for traditional cryoprotection and X-ray diffraction data collection. Protein crystals grown in these plastic devices can be directly subjected to in situ X-ray diffraction studies. The MPCS integrates the formulation of crystallization cocktails with the preparation of the crystallization experiments. Within microfluidic Teflon tubing or the microfluidic circuitry of a plastic CrystalCard, ~10-20 nl volume droplets are generated, each representing a microbatch-style crystallization experiment with a different chemical composition. The entire protein sample is utilized in crystallization experiments. Sparse-matrix screening and chemical gradient screening can be combined in one comprehensive `hybrid' crystallization trial. The technology lends itself well to optimization by high-granularity gradient screening using optimization reagents such as precipitation agents, ligands or cryoprotectants. | en_US |
dc.publisher | International Union of Crystallography | en_US |
dc.subject | Protein crystallization | en_US |
dc.subject | Microcapillary protein crystallization system | en_US |
dc.title | The plug-based nanovolume Microcapillary Protein Crystallization System (MPCS) | en_US |
dc.type | Article | |
kusw.kuauthor | Lovell, Scott W. | |
kusw.kudepartment | Higuchi Biosciences Center | en_US |
dc.identifier.doi | 10.1107/S0907444908028060 | |
kusw.oaversion | Scholarly/refereed, publisher version | |
kusw.oapolicy | This item does not meet KU Open Access policy criteria. | |
dc.rights.accessrights | openAccess | |