The plug-based nanovolume Microcapillary Protein Crystallization System (MPCS)
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Issue Date
2008Author
Gerdts, Cory. J
Elliot, Mark
Lovell, Scott
Mixon, Mark B.
Napuli, Alberto J.
Staker, Bart L.
Nollert, Peter
Stewart, Lance
Publisher
International Union of Crystallography
Type
Article
Article Version
Scholarly/refereed, publisher version
Metadata
Show full item recordAbstract
The Microcapillary Protein Crystallization System (MPCS) embodies a new semi-automated plug-based crystallization technology which enables nanolitre-volume screening of crystallization conditions in a plasticware format that allows crystals to be easily removed for traditional cryoprotection and X-ray diffraction data collection. Protein crystals grown in these plastic devices can be directly subjected to in situ X-ray diffraction studies. The MPCS integrates the formulation of crystallization cocktails with the preparation of the crystallization experiments. Within microfluidic Teflon tubing or the microfluidic circuitry of a plastic CrystalCard, ~10-20 nl volume droplets are generated, each representing a microbatch-style crystallization experiment with a different chemical composition. The entire protein sample is utilized in crystallization experiments. Sparse-matrix screening and chemical gradient screening can be combined in one comprehensive `hybrid' crystallization trial. The technology lends itself well to optimization by high-granularity gradient screening using optimization reagents such as precipitation agents, ligands or cryoprotectants.
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This is the published version. Copyright International Union of Crystallography
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Citation
Gerdts, Cory J., Mark Elliott, Scott Lovell, Mark B. Mixon, Alberto J. Napuli, Bart L. Staker, Peter Nollert, and Lance Stewart. "The Plug-based Nanovolume Microcapillary Protein Crystallization System (MPCS)." Acta Crystallogr D Biol Cryst Acta Cryst D Acta Crystallogr Sect D Biol Crystallogr Acta Crystallogr Sect D Acta Crystallogr D Biol Crystallogr Acta Crystallographica Section D Biological Crystallography Acta Cryst Sect D Acta Crystallogr D 64.11 (2008): 1116-122. http//dx.doi.org/10.1107/S0907444908028060
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