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    The plug-based nanovolume Microcapillary Protein Crystallization System (MPCS)

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    gerdts_protein_2008.pdf (1.414Mb)
    Issue Date
    2008
    Author
    Gerdts, Cory. J
    Elliot, Mark
    Lovell, Scott
    Mixon, Mark B.
    Napuli, Alberto J.
    Staker, Bart L.
    Nollert, Peter
    Stewart, Lance
    Publisher
    International Union of Crystallography
    Type
    Article
    Article Version
    Scholarly/refereed, publisher version
    Metadata
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    Abstract
    The Microcapillary Protein Crystallization System (MPCS) embodies a new semi-automated plug-based crystallization technology which enables nanolitre-volume screening of crystallization conditions in a plasticware format that allows crystals to be easily removed for traditional cryoprotection and X-ray diffraction data collection. Protein crystals grown in these plastic devices can be directly subjected to in situ X-ray diffraction studies. The MPCS integrates the formulation of crystallization cocktails with the preparation of the crystallization experiments. Within microfluidic Teflon tubing or the microfluidic circuitry of a plastic CrystalCard, ~10-20 nl volume droplets are generated, each representing a microbatch-style crystallization experiment with a different chemical composition. The entire protein sample is utilized in crystallization experiments. Sparse-matrix screening and chemical gradient screening can be combined in one com­prehensive `hybrid' crystallization trial. The technology lends itself well to optimization by high-granularity gradient screening using optimization reagents such as precipitation agents, ligands or cryoprotectants.
    Description
    This is the published version. Copyright International Union of Crystallography
    URI
    http://hdl.handle.net/1808/18969
    DOI
    https://doi.org/10.1107/S0907444908028060
    Collections
    • Higuchi Biosciences Center Scholarly Works [47]
    Citation
    Gerdts, Cory J., Mark Elliott, Scott Lovell, Mark B. Mixon, Alberto J. Napuli, Bart L. Staker, Peter Nollert, and Lance Stewart. "The Plug-based Nanovolume Microcapillary Protein Crystallization System (MPCS)." Acta Crystallogr D Biol Cryst Acta Cryst D Acta Crystallogr Sect D Biol Crystallogr Acta Crystallogr Sect D Acta Crystallogr D Biol Crystallogr Acta Crystallographica Section D Biological Crystallography Acta Cryst Sect D Acta Crystallogr D 64.11 (2008): 1116-122. http//dx.doi.org/10.1107/S0907444908028060

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    KU Libraries
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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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