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dc.contributor.authorChakraborty, Aishik
dc.contributor.authorHui, Erica
dc.contributor.authorWaring, Alan J.
dc.contributor.authorDhar, Prajnaparamita
dc.date.accessioned2015-10-19T14:56:34Z
dc.date.available2015-10-19T14:56:34Z
dc.date.issued2015-01-27
dc.identifier.citationChakraborty, Aishik, Erica Hui, Alan J. Waring, and Prajnaparamita Dhar. "Tug of War in Lung Surfactant Components: MiniB Dominates over Cholesterol during Lipid Domain Formation." Biophysical Journal 108.2 (2015): n. pag. http://dx.doi.org/10.1016/j.bpj.2014.11.2109en_US
dc.identifier.urihttp://hdl.handle.net/1808/18704
dc.descriptionThis is the published version. Copyright © 2015 Biophysical Society. Published by Elsevier Inc. All rights reserved.en_US
dc.description.abstractLung surfactants (LS), a complex mixture of lipids and proteins present in the alveolar lining of lungs, help in lowering surface tension to near zero at expiration. Deficiency of this surfactant can lead to Neonatal Respiratory Distress Syndrome in infants, while a dysfunction of LS can cause Acute Respiratory Distress Syndrome (ARDS) that affects patients irrespective of age. Successful medical intervention such as surfactant replacement therapy (SRT) requires a good understanding of surfactant composition and function. Currently there is no consensus on the composition of LS used in SRT, particularly the interactions between components making up this mixture. Our objective was to understand the interaction of cholesterol (a component whose role and even presence in SRT is highly debated) and MiniB (a synthetic protein mimic of native surfactant protein SP-B) at air-water interface. We report the alteration in lipid domain formation of films containing 1,2-dipalmitoyl- sn- glycero- 3- phosphocholine (DPPC): 1- palmitoyl- 2- oleoyl- sn- glycero- 3- phosphatidylglycerol (POPG) in the ratio 7:3 under the influence of varying concentrations of MiniB and cholesterol. Fluorescence imaging under constant compression, along with analysis of domain size distributions, reveals that MiniB increases line tension between lipid domains, and prefers to stay in fluid POPG regions, making the liquid-ordered domains smaller in size. Small amounts of cholesterol prefer packed domains, stretching them into spirals during the process, lowering their line tension. In both cases, higher concentration yields more prominent consequences in terms of the stated changes. However, mixture containing both cholesterol and MiniB shows reduction in domain size with no changes in domain shape. This suggests the dominance of MiniB over cholesterol when interacting with lipid domains, which may have important effects on the performance of synthetic LS.en_US
dc.publisherElsevieren_US
dc.titleTug of War in Lung Surfactant Components: MiniB Dominates over Cholesterol during Lipid Domain Formationen_US
dc.typeArticle
kusw.kuauthorDhar, Prajna
kusw.kudepartmentChemical & Petroleum Engren_US
dc.identifier.doi10.1016/j.bpj.2014.11.2109
kusw.oaversionScholarly/refereed, publisher version
kusw.oapolicyThis item meets KU Open Access policy criteria.
dc.rights.accessrightsopenAccess


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