Cost effective filamentous phage based immunization nanoparticles displaying a full-length hepatitis B virus surface antigen
dc.contributor.author | Baclioglu, Bertan Koray | |
dc.contributor.author | Ozdemir-Bahadir, Aylin | |
dc.contributor.author | Hinc, Duygu | |
dc.contributor.author | Erdag, Berrin | |
dc.contributor.author | Tamerler, Candan | |
dc.date.accessioned | 2015-10-16T17:32:35Z | |
dc.date.available | 2015-10-16T17:32:35Z | |
dc.date.issued | 2014 | |
dc.identifier.citation | Balcioglu, Bertan Koray, Aylin Ozdemir-Bahadir, Duygu Hinc, Candan Tamerler, and Berrin Erdag. "Cost Effective Filamentous Phage Based Immunization Nanoparticles Displaying a Full-length Hepatitis B Virus Surface Antigen." Advances in Bioscience and Biotechnology ABB 05.01 (2014): 46-53. doi:10.4236/abb.2014.51008. | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/18700 | |
dc.description.abstract | Hepatitis B virus (HBV) is one of the major causes of chronic hepatitis, cirrhosis and liver cancer. In combating HBV infections, HBV diagnosis and vaccination are therefore critical. The hepatitis B virus surface antigen (HBsAg) is a key target molecule in developing vaccines and diagnostic systems. To date, although HBsAg has been expressed in bacteria, yeasts and mammalian cells, there are still limitations in the existing ones, which leave the necessity for searching new HBsAg production methods. In this study, a simple phage display-based method was developed to produce the purified full-length HBsAg molecules for further immunization studies. For this purpose, the HBsAg coding gene was cloned into a pCANTAB5E phagemid vector and expressed on the surface of M13 filamentous phages. The HBsAg-expressing phage nanosystem was then used as immunization agent in BALB/cJ mice. The ELISA results for sera obtained from mice immunized with HBsAg-displaying phage particles revealed an immune response against HBsAg. These results demonstrate the potential use of a full-length antigen to be displayed on phages as cost effective adjuvant-free immunization agents as an alternative to the highly purified and more expensive antigens conjugated with carrier molecules. | en_US |
dc.publisher | Scientific Research Publishing | en_US |
dc.rights | Copyright © 2014 Bertan Koray Balcioglu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. In accordance of the Creative Commons Attribution License all Copyrights © 2014 are reserved for SCIRP and the owner of the intellectual property Bertan Koray Balcioglu et al. All Copyright © 2014 are guarded by law and by SCIRP as a guardian. | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.title | Cost effective filamentous phage based immunization nanoparticles displaying a full-length hepatitis B virus surface antigen | en_US |
dc.type | Article | |
kusw.kuauthor | Tamerler, Candan | |
kusw.kudepartment | Mechanical Engineering | en_US |
dc.identifier.doi | 10.4236/abb.2014.51008 | |
kusw.oaversion | Scholarly/refereed, publisher version | |
kusw.oapolicy | This item does not meet KU Open Access policy criteria. | |
dc.rights.accessrights | openAccess |
Files in this item
This item appears in the following Collection(s)
Except where otherwise noted, this item's license is described as: Copyright © 2014 Bertan Koray Balcioglu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. In accordance of the Creative Commons Attribution License all Copyrights © 2014 are reserved for SCIRP and the owner of the intellectual property Bertan Koray Balcioglu et al. All Copyright © 2014 are guarded by law and by SCIRP as a guardian.