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    The Structure of DNA within Cationic Lipid/DNA Complexes

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    MiddaughC_BJ_84(2)1114.pdf (321.0Kb)
    Issue Date
    2003-02
    Author
    Braun, Chad S.
    Jas, Gouri S.
    Choosakoonriang, Sirirat
    Koe, Gary S.
    Smith, Janet G.
    Middaugh, C. Russell
    Publisher
    Biophysical Society
    Type
    Article
    Article Version
    Scholarly/refereed, publisher version
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    Abstract
    The structure of DNA within CLDCs used for gene delivery is controversial. Previous studies using CD have been interpreted to indicate that the DNA is converted from normal B to C form in complexes. This investigation reexamines this interpretation using CD of model complexes, FTIR as well as Raman spectroscopy and molecular dynamics simulations to address this issue. CD spectra of supercoiled plasmid DNA undergo a significant loss of rotational strength in the signal near 275 nm upon interaction with either the cationic lipid dimethyldioctadecylammonium bromide or 1,2-dioleoyltrimethylammonium propane. This loss of rotational strength is shown, however, by both FTIR and Raman spectroscopy to occur within the parameters of the B-type conformation. Contributions of absorption flattening and differential scattering to the CD spectra of complexes are unable to account for the observed spectra. Model studies of the CD of complexes prepared from synthetic oligonucleotides of varying length suggest that significant reductions in rotational strength can occur within short stretches of DNA. Furthermore, some alteration in the hydrogen bonding of bases within CLDCs is indicated in the FTIR and Raman spectroscopy results. In addition, alterations in base stacking interactions as well as hydrogen bonding are suggested by molecular dynamics simulations. A global interpretation of all of the data suggests the DNA component of CLDCs remains in a variant B form in which base/base interactions are perturbed.
    URI
    http://hdl.handle.net/1808/17668
    DOI
    https://doi.org/10.1016/S0006-3495(03)74927-3
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    • Pharmaceutical Chemistry Scholarly Works [327]
    Citation
    Braun et. al. "The Structure of DNA within Cationic Lipid/DNA Complexes." Volume 84, Issue 2, February 2003, Pages 1114–1123. http://dx.doi.org/10.1016/S0006-3495(03)74927-3

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    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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