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dc.contributor.authorBraun, Chad S.
dc.contributor.authorFisher, Mark T.
dc.contributor.authorTomalia, Donald A.
dc.contributor.authorKoe, Gary S.
dc.contributor.authorKoe, Janet G.
dc.contributor.authorMiddaugh, C. Russell
dc.date.accessioned2015-05-08T19:15:21Z
dc.date.available2015-05-08T19:15:21Z
dc.date.issued2005-06
dc.identifier.citationBraun et. al. "A Stopped-Flow Kinetic Study of the Assembly of Nonviral Gene Delivery Complexes." Volume 88, Issue 6, June 2005, Pages 4146–4158.

http://dx.doi.org/10.1529/biophysj.104.055202
en_US
dc.identifier.urihttp://hdl.handle.net/1808/17666
dc.description.abstractStopped-flow circular dichroism and fluorescence spectroscopy are used to characterize the assembly of complexes consisting of plasmid DNA bound to the cationic lipids dimethyldioctadecylammonium bromide and 1, 2-dioleoyl- 3- trimethylammonium-propane and a series of polyamidoamine dendrimers. The kinetics of complexation determined from the stopped-flow circular dichroism measurements suggests complexation occurs within 50 ms. Further analysis, however, was precluded by the presence of mixing (shear) artifacts. Stopped-flow fluorescence employing the high-affinity DNA dyes Hoechst 33258 and YOYO-1 was able to resolve two sequential steps in the assembly of complexes that are assigned to binding/ dehydration and condensation events. The rates of each process were determined over the temperature range of 10–50 C and activation energies were determined from the slope of Arrhenius plots. The behavior of polyamidoamine dendrimers can be separated into two classes based on their differing binding modes: generation 2 and the larger generations (G4, G7, and G9). The larger generations have activation energies for binding that follow the trend G4 . G7 . G9. The activation energies for condensation (compaction) of complexes composed of these same dendrimers have the opposite trend G9 . G7 . G4. It is postulated that a balance between a more energetically favorable condensation and less favorable binding may prove beneficial in enhancing gene delivery.en_US
dc.publisherBiophysical Societyen_US
dc.titleA Stopped-Flow Kinetic Study of the Assembly of Nonviral Gene Delivery Complexesen_US
dc.typeArticle
kusw.kuauthorBraun, Chad S.
kusw.kuauthorFisher, Mark T.
kusw.kuauthorMiddaugh, C. Russell
kusw.kudepartmentDepartment of Pharmaceutical Chemistyen_US
dc.identifier.doi10.1529/biophysj.104.055202
kusw.oaversionScholarly/refereed, publisher version
kusw.oapolicyThis item does not meet KU Open Access policy criteria.
dc.rights.accessrightsopenAccess


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