Two-photon microscopy with wavelength switchable fiber laser excitation
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Issue Date
2006-10-16Author
Unruh, Jay R.
Price, E. Shane
Molla, Roque Gagliano
Stehno-Bittel, Lisa
Johnson, Carey K.
Hui, Rongqing
Publisher
Optical Society of America
Type
Article
Article Version
Scholarly/refereed, publisher version
Metadata
Show full item recordAbstract
Two-photon scanning fluorescence microscopy has become a powerful tool for imaging living cells and tissues. Most applications of two-photon microscopy employ a Ti:sapphire laser excitation source, which is not readily portable or rapidly tunable. This work explores the use of two-photon fiber laser excitation (TP-FLEX) as an excitation source for scanning two-photon microscopy. We have further demonstrated the use of a photonic crystal fiber (PCF) for facile tuning of the excitation wavelength over the range from 810 nm to 1100 nm. We generated two-photon fluorescence images at excitation wavelengths from 850 nm to 1100 nm detected on a scanning-stage microscope. By PCF wavelength tuning the dye BODIPY fl was selectively excited at 1000 nm whereas MitoTracker red was excited preferentially at 1100 nm. We discuss the potential for fiber laser sources coupled with PCF wavelength tuning as an attractive tunable excitation source for two-photon scanning fluorescence microscopy.
Description
This is the publisher's version, also available electronically from http://www.opticsinfobase.org/oe/abstract.cfm?uri=oe-14-21-9825.
ISSN
1094-4087Collections
Citation
Unruh, Jay R. et al. (2006). "Two-photon microscopy with wavelength switchable fiber laser excitation." Optics Express, 14(21):9825-9831. http://dx.doi.org/10.1364/OE.14.009825.
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