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    Single molecule detection and underwater fluorescence imaging with cantilevered near-field fiber optic probes

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    DunnR_1998.pdf (357.6Kb)
    Issue Date
    1998-04-03
    Author
    Talley, Chad E.
    Lee, M. Annie
    Dunn, Robert C.
    Publisher
    American Institute of Physics
    Type
    Article
    Article Version
    Scholarly/refereed, publisher version
    Metadata
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    Abstract
    Tapping-mode near-field scanning optical microscopy (NSOM) employing a cantilevered fiber optic probe is utilized to image the fluorescence from single molecules and samples in aqueous environments. The single molecule fluorescenceimages demonstrate both the subdiffraction limit spatial resolution and low detection limit capabilities of the cantilevered probe design. Images taken as a function of tip oscillation drive amplitude reveal a degradation in the resolution as the amplitude is increased. With all cantilevered probes studied, however, a minimum plateau region in the resolution is reached as the drive amplitude is decreased, indicating that the tapping mode of operation does not reduce the optical resolution. Images of fluorescently dopedlipid films illustrate the ability of the probe to track small height changes (<1.5 nm) in ambient and aqueous environments, while maintaining high resolution in the fluorescenceimage. When the tip is immersed in water (1.3 mm), the cantilevered NSOM tip resonance, 25–50 kHz, shifts approximately 100–150 Hz, the amplitude dampens less than 40% and the Q factor is reduced from 300–500 to 100–200.
    Description
    This is the published version, also available here: http://dx.doi.org/10.1063/1.121505.
    URI
    http://hdl.handle.net/1808/16049
    DOI
    https://doi.org/10.1063/1.121505
    Collections
    • Chemistry Scholarly Works [610]
    Citation
    Talley, Chad E., Lee, M. Annie., Dunn, Robert C. "Single molecule detection and underwater fluorescence imaging with cantilevered near-field fiber optic probes." Appl. Phys. Lett. 72, 2954 (1998); http://dx.doi.org/10.1063/1.121505.

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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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