Hybrid near-field scanning optical microscopy tips for live cell measurements

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Issue Date
2004-03-08Author
Kapkiai, Luka K.
Moore-Nichols, David
Carnell, Jonathan
Krogmeier, Jeffrey R.
Dunn, Robert C.
Publisher
American Institute of Physics
Type
Article
Article Version
Scholarly/refereed, publisher version
Metadata
Show full item recordAbstract
We report a near-field scanning optical microscopy (NSOM) probe that enables high-resolution imaging of living cells under physiological buffered conditions. The hybrid design combines a conventional fiber optic near-field probe with a standard atomic force microscopy cantilever. Imaging of fluorescent latex spheres suspended in an acetate matrix demonstrates the subdiffraction limited fluorescence and topography capabilities of the tips. The reduced spring constant of the hybrid tip is also shown to be amenable to measurements on living cells. NSOM fluorescence and topography measurements on living human arterial smooth muscle cells under buffered conditions are demonstrated.
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This is the published version, also available here: http://dx.doi.org/10.1063/1.1737464.
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Citation
Kapkiai, Luka K., Moore-Nichols, David., Carnell, Jonathan., Krogmeier, Jeffrey R., Dunn, Robert C. "Hybrid near-field scanning optical microscopy tips for live cell measurements." Appl. Phys. Lett. 84, 3750 (2004); http://dx.doi.org/10.1063/1.1737464.
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