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1.15 Å resolution structure of the proteasome-assembly chaperone Nas2 PDZ domain

dc.contributor.authorSingh, Chingakham Ranjit
dc.contributor.authorLovell, Scott
dc.contributor.authorMehzabeen, Nurjahan
dc.contributor.authorChowdhury, Wasimul Q.
dc.contributor.authorGeanes, Eric S.
dc.contributor.authorBattaile, Kevin P.
dc.contributor.authorRoelofs, Jeroen
dc.date.accessioned2014-05-01T19:42:58Z
dc.date.available2014-05-01T19:42:58Z
dc.date.issued2014-04-01
dc.identifier.citationSingh, C. R., Lovell, S., Mehzabeen, N., Chowdhury, W. Q., Geanes, E. S., Battaile, K. P. & Roelofs, J. (2014). 1.15 Å resolution structure of the proteasome-assembly chaperone Nas2 PDZ domain. Acta Cryst. F70, 418-423. http://dx.doi.org/10.1107/S2053230X14003884
dc.identifier.urihttp://hdl.handle.net/1808/13617
dc.description.abstractThe 26S proteasome is a 2.5 MDa protease dedicated to the degradation of ubiquitinated proteins in eukaryotes. The assembly of this complex containing 66 polypeptides is assisted by at least nine proteasome-specific chaperones. One of these, Nas2, binds to the proteasomal AAA-ATPase subunit Rpt5. The PDZ domain of Nas2 binds to the C-terminal tail of Rpt5; however, it does not require the C-terminus of Rpt5 for binding. Here, the 1.15 Å resolution structure of the PDZ domain of Nas2 is reported. This structure will provide a basis for further insights regarding the structure and function of Nas2 in proteasome assembly.
dc.description.sponsorshipThis work was supported by grants from the Johnson Cancer Research Center, the NIH-NCRR (5P20RR017708 and P20 RR016475) and NIH (8 P20 GM103420 and P20 GM103418). Use of IMCA-CAT was supported by IMCA though a contract with the Hauptman-Woodward MRI. Use of the APS was supported by the US Department of Energy (Contract No. DE-AC02-06CH11357). Publication of this article was funded in part by the Kansas State University Open Access Publishing Fund.
dc.publisherStructural Biology Communications
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectNas2
dc.subjectchaperones
dc.subjectProteasome
dc.subjectPDZ domain
dc.title1.15 Å resolution structure of the proteasome-assembly chaperone Nas2 PDZ domain
dc.typeArticle
kusw.kuauthorLovell, Scott
kusw.kuauthorMehzabeen, Nurjahan
kusw.kudepartmentProtien Structure Lab
kusw.oastatusfullparticipation
dc.identifier.doi10.1107/S2053230X14003884
kusw.oaversionScholarly/refereed, publisher version
kusw.oapolicyThis item meets KU Open Access policy criteria.
dc.rights.accessrightsopenAccess


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This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
Except where otherwise noted, this item's license is described as: This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.