Mi2β Is Required for γ-Globin Gene Silencing: Temporal Assembly of a GATA-1-FOG-1-Mi2 Repressor Complex in β-YAC Transgenic Mice
dc.contributor.author | Costa, Flávia C. | |
dc.contributor.author | Fedosyuk, Halyna | |
dc.contributor.author | Chazelle, Allen M. | |
dc.contributor.author | Neades, Renee Y. | |
dc.contributor.author | Peterson, Kenneth R. | |
dc.date.accessioned | 2013-07-29T19:45:55Z | |
dc.date.available | 2013-07-29T19:45:55Z | |
dc.date.issued | 2012-12-20 | |
dc.identifier.citation | Costa, Flavia C.; Fedosyuk, Halyna; Chazelle, Allen M.; Neades, Renee Y.; and Peterson, Kenneth R. (2012) Mi2β Is Required for γ-Globin Gene Silencing: Temporal Assembly of a GATA-1-FOG-1-Mi2 Repressor Complex in β-YAC Transgenic Mice. Plos Genetics, 8.12. http://dx.doi.org/10.1371/journal.pgen.1003155 | |
dc.identifier.uri | http://hdl.handle.net/1808/11534 | |
dc.description | A grant from the One-University Open Access Fund at the University of Kansas was used to defray the author’s publication fees in this Open Access journal. The Open Access Fund, administered by librarians from the KU, KU Law, and KUMC libraries, is made possible by contributions from the offices of KU Provost, KU Vice Chancellor for Research & Graduate Studies, and KUMC Vice Chancellor for Research. For more information about the Open Access Fund, please see http://library.kumc.edu/authors-fund.xml. | |
dc.description.abstract | Activation of γ-globin gene expression in adults is known to be therapeutic for sickle cell disease. Thus, it follows that the converse, alleviation of repression, would be equally effective, since the net result would be the same: an increase in fetal hemoglobin. A GATA-1-FOG-1-Mi2 repressor complex was recently demonstrated to be recruited to the −566 GATA motif of the Aγ-globin gene. We show that Mi2β is essential for γ-globin gene silencing using Mi2β conditional knockout β-YAC transgenic mice. In addition, increased expression of Aγ-globin was detected in adult blood from β-YAC transgenic mice containing a T>G HPFH point mutation at the −566 GATA silencer site. ChIP experiments demonstrated that GATA-1 is recruited to this silencer at day E16, followed by recruitment of FOG-1 and Mi2 at day E17 in wild-type β-YAC transgenic mice. Recruitment of the GATA-1–mediated repressor complex was disrupted by the −566 HPFH mutation at developmental stages when it normally binds. Our data suggest that a temporal repression mechanism is operative in the silencing of γ-globin gene expression and that either a trans-acting Mi2β knockout deletion mutation or the cis-acting −566 Aγ-globin HPFH point mutation disrupts establishment of repression, resulting in continued γ-globin gene transcription during adult definitive erythropoiesis. | |
dc.language.iso | en_US | |
dc.publisher | Public Library of Science | |
dc.rights | Copyright ©2012 Costa et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.title | Mi2β Is Required for γ-Globin Gene Silencing: Temporal Assembly of a GATA-1-FOG-1-Mi2 Repressor Complex in β-YAC Transgenic Mice | |
dc.type | Article | |
kusw.kuauthor | Peterson, Kenneth R. | |
kusw.kudepartment | Department of Biochemistry and Molecular Biology | |
kusw.oanotes | The processing fees for this article were funded by The One - University Open Access Fund. | |
kusw.oastatus | fullparticipation | |
dc.identifier.doi | 10.1371/journal.pgen.1003155 | |
dc.identifier.orcid | https://orcid.org/0000-0001-9990-3303 | |
kusw.oaversion | Scholarly/refereed, publisher version | |
kusw.oapolicy | This item meets KU Open Access policy criteria. | |
dc.rights.accessrights | openAccess |
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Except where otherwise noted, this item's license is described as: Copyright ©2012 Costa et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.