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    Host identity impacts rhizosphere fungal communities associated with three alpine plant species

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    Main text including tables and figures (762.1Kb)
    Sequence alignment and neighbor-joining tree for the full dataset including AMF OTUs, non-AMF OTUs, and reference sequences. (24.10Kb)
    Sequence alignment and neighbor-joining tree for the AMF dataset including AMF OTUs, reference sequences, and outgroup taxa. NOTE: This file will open in a browser window. To save a copy, right-click on the link for "Appendix B.txt" and select the option to save the file from the resulting menu. (24.10Kb)
    GenBank accession number, top BLASTN match, and phylogenetic assignment for each OTU. (305.4Kb)
    Number of sequences, number of OTUs, and relative abundance of each OTU per root sample. (543.0Kb)
    AMF richness, diversity, and evenness per root sample based on OTUs designated at 90%, 95%, 97%, and 99% sequence similarity. (353.6Kb)
    Non-AMF richness, diversity, and evenness per root sample based on OTUs designated at 90%, 95%, 97%, and 99% sequence similarity. (302.4Kb)
    Issue Date
    2012
    Author
    Becklin, Katie M.
    Hertweck, Kate L.
    Jumpponen, Ari
    Publisher
    Springer Verlag
    Type
    Article
    Article Version
    Scholarly/refereed, author accepted manuscript
    Metadata
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    Abstract
    Fungal diversity and composition are still relatively unknown in many ecosystems; however, host identity and environmental conditions are hypothesized to influence fungal community assembly. To test these hypotheses we characterized the richness, diversity, and composition of rhizosphere fungi colonizing three alpine plant species, Taraxacum ceratophorum, Taraxacum officinale, and Polemonium viscosum. Roots were collected from open meadow and willow understory habitats at treeline on Pennsylvania Mountain, Colorado, USA. Fungal small subunit ribosomal DNA was sequenced using fungal-specific primers, sample-specific DNA tags, and 454 pyrosequencing. We classified operational taxonomic units (OTUs) as arbuscular mycorrhizal (AMF) or non-arbuscular mycorrhizal (non-AMF) fungi, then tested whether habitat or host identity influenced these fungal communities. Approximately 14% of the sequences represented AMF taxa (44 OTUs) with the majority belonging to Glomus group A and B. NON-AMF sequences represented 186 OTUs belonging to Ascomycota (58%), Basidiomycota (26%), Zygomycota (14%), and Chytridiomycota (2%) phyla. Total AMF and non-AMF richness were similar between habitats, but varied among host species. AMF richness and diversity per root sample also varied among host species and were highest in T. ceratophorum compared to T. officinale and P. viscosum. In contrast, non-AMF richness and diversity per root sample were similar among host species except in the willow understory where diversity was reduced in T. officinale. Fungal community composition was influenced by host identity, but not habitat. Specifically, T. officinale hosted a different AMF community than T. ceratophorum and P. viscosum, while P. viscosum hosted a different non-AMF community than T. ceratophorum and T. officinale. Our results suggest that host identity has a stronger effect on rhizosphere fungi than habitat. Furthermore, although host identity influenced both AMF and non-AMF this effect was stronger for the mutualistic AMF community.
    Description
    This is the author’s accepted manuscript. The original publication is available at www.springerlink.com. For the publisher’s version go to http://dx.doi.org/10.1007/s00248-011-9968-7. NOTE: The supplementary materials referred to in the text are available for download separately, below.
    URI
    http://hdl.handle.net/1808/8740
    DOI
    https://doi.org/10.1007/s00248-011-9968-7
    Collections
    • Ecology & Evolutionary Biology Scholarly Works [1516]
    Citation
    Becklin KM, Hertweck KL, and Jumpponen A. 2012. Host identity impacts rhizosphere fungal communities associated with three alpine plant species. Microbial Ecology 63:682-693. http://dx.doi.org/10.1007/s00248-011-9968-7

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    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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