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dc.contributor.advisorHui, Rongqing
dc.contributor.authorLoganathan, Rajprasad
dc.date.accessioned2011-10-09T03:42:42Z
dc.date.available2011-10-09T03:42:42Z
dc.date.issued2011-07-31
dc.date.submitted2011
dc.identifier.otherhttp://dissertations.umi.com/ku:11638
dc.identifier.urihttp://hdl.handle.net/1808/8152
dc.description.abstractFibronectin is a major extracellular matrix molecular component that plays a critical role in embryonic cell motility and morphogenesis. During early development, the fibronectin molecular network is nearly ubiquitous in distribution across the entire embryonic volume. As a result, the embryonic fibronectin distribution is functionally relevant to both cell motility and organogenesis. Despite its biological importance, the structural attributes of embryonic fibronectin distribution are poorly understood. The textural features of an extracellular matrix network like that of fibronectin is not known. By marking the embryonic tissue, obtained during specific stages of development, using fibronectin indirect immunofluorescence, fluorescent microscopy images capturing the embryonic fibronectin distribution were obtained. Using the properties of the gray scale co-occurrence matrix, textural attributes of a fibronectin network was derived for quail embryos during their early stages of morphogenesis. As a result, textural properties like inertia, correlation, uniformity, entropy and homogeneity were assessed for the medial (including the embryonic anteroposterior axis) and lateral (excluding the embryonic anteroposterior axis) fibronectin . The results not only demonstrated a noticeable heterogeneity of fibronectin textural properties across the embryonic regions examined, but also across the developmental stages that were studied. The spatial anisotropy and the temporal evolution across the developmental span in the textural attributes of embryonic fibronectin may have functional consequences for cell motility and morphogenesis. With this thesis, textural analysis has found yet another application, viz. the study of the surface structural attributes of an extracellular matrix protein network in the context of developmental biology. The work presented in this thesis is the first of its kind in applying texture analysis with gray scale co-occurrence matrix method to study the spatial distribution of fibronectin matrix within a given stage of embryonic development and the temporal textural changes as the embryonic development progresses from stage 5 through stage 9.
dc.format.extent86 pages
dc.language.isoen
dc.publisherUniversity of Kansas
dc.rightsThis item is protected by copyright and unless otherwise specified the copyright of this thesis/dissertation is held by the author.
dc.subjectElectrical engineering
dc.subjectDevelopmental biology
dc.subjectExtracellular matrix
dc.subjectGray scale co-occurrence
dc.titleTextural Analysis of a Fibronectin Network During Early Embryogenesis
dc.typeThesis
dc.contributor.cmtememberChakrabarti, Swapan
dc.contributor.cmtememberPotetz, Brian
dc.contributor.cmtememberCzirok, Andras
dc.thesis.degreeDisciplineElectrical Engineering & Computer Science
dc.thesis.degreeLevelM.S.
kusw.oastatusna
kusw.oapolicyThis item does not meet KU Open Access policy criteria.
kusw.bibid7642896
dc.rights.accessrightsopenAccess


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