KUKU

KU ScholarWorks

  • myKU
  • Email
  • Enroll & Pay
  • KU Directory
    • Login
    View Item 
    •   KU ScholarWorks
    • Dissertations and Theses
    • Theses
    • View Item
    •   KU ScholarWorks
    • Dissertations and Theses
    • Theses
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Adenoviral Mediated Gene Delivery to Human Umbilical Cord Mesenchymal Stromal Cells for Inner Ear Hair Cell Differentiation

    Thumbnail
    View/Open
    Devarajan_ku_0099M_11665_DATA_1.pdf (6.488Mb)
    Issue Date
    2011-07-28
    Author
    Devarajan, Keerthana
    Publisher
    University of Kansas
    Format
    106 pages
    Type
    Thesis
    Degree Level
    M.S.
    Discipline
    Bioengineering
    Rights
    This item is protected by copyright and unless otherwise specified the copyright of this thesis/dissertation is held by the author.
    Metadata
    Show full item record
    Abstract
    Hearing is one of our main sensory systems and having a hearing disorder, although not life threatening, can have a disturbing impact in an individual's quality of life. Approximately 49 million Americans suffer from some form of hearing loss. Sensory neural hearing loss (SNHL) is the most common form, which results from degeneration of inner ear sensory hair cells and auditory neurons in the cochlea. In recent years, there has been an increasing interest in gene delivery to mesenchymal stem cells. Gene delivery approaches to stem cells can provide an opportunity to engineer a variety of specialized cell types. The objective of this thesis was to evaluate the potential of human umbilical cord mesenchymal stromal cells (hUCMSCs) as a possible source for regenerating inner ear hair cells. This thesis was successful in developing an adenoviral mediated gene delivery approach to deliver the Math1 gene to hUCMSCs. The expression of Math1 induced the differentiation of hUCMSCs into cells that resembled inner ear hair cells morphologically and immunocytochemically, evidenced by the expression of hair cell-specific and glial cell markers. The results obtained in this thesis demonstrated for the first time that hUCMSCs can differentiate into hair cell-like cells, thus introducing a potential tissue engineering and cell transplantation approach for the treatment of hearing loss.
    URI
    http://hdl.handle.net/1808/8056
    Collections
    • Engineering Dissertations and Theses [1055]
    • Theses [3828]

    Items in KU ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.


    We want to hear from you! Please share your stories about how Open Access to this item benefits YOU.


    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

    Browse

    All of KU ScholarWorksCommunities & CollectionsThis Collection

    My Account

    LoginRegister

    Statistics

    View Usage Statistics

    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

    The University of Kansas
      Contact KU ScholarWorks
    Lawrence, KS | Maps
     
    • Academics
    • Admission
    • Alumni
    • Athletics
    • Campuses
    • Giving
    • Jobs

    The University of Kansas prohibits discrimination on the basis of race, color, ethnicity, religion, sex, national origin, age, ancestry, disability, status as a veteran, sexual orientation, marital status, parental status, gender identity, gender expression and genetic information in the University’s programs and activities. The following person has been designated to handle inquiries regarding the non-discrimination policies: Director of the Office of Institutional Opportunity and Access, IOA@ku.edu, 1246 W. Campus Road, Room 153A, Lawrence, KS, 66045, (785)864-6414, 711 TTY.

     Contact KU
    Lawrence, KS | Maps