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    RNA Helicase 1 interacts with an ABCRNAi Transporter: Genetic Interactions with haf-6

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    Issue Date
    2010-12-03
    Author
    Rivera, Laticia
    Publisher
    University of Kansas
    Format
    47 pages
    Type
    Thesis
    Degree Level
    M.A.
    Discipline
    Molecular Biosciences
    Rights
    This item is protected by copyright and unless otherwise specified the copyright of this thesis/dissertation is held by the author.
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    Abstract
    The C. elegans rha-1 gene encodes a conserved helicase with ATP-dependent DEAD/H-box and double-stranded RNA binding domains. rha-1 is orthologous to the Drosophila maleless gene(MLE), an essential component of the dosage compensation machinery that leads to a two-fold increase in transcription of genes located on the single X chromosome of males in comparison to the transcription rate of homologous genes located on an X chromosome in females. The human ortholog, RNA helicase A (RHA), unwinds double-stranded DNA and RNA in a 3' to 5' direction. RHA is a component of several distinct protein complexes, including the RNA-induced silencing complex (RISC), the coding region determinant (CRD)-mediated complex, mRNP granules, and also associates with BRCA1, CREBbp or SMN1 and the RNA polymerase II complex, phosphorylated histones (H2AFX). RHA affects a number of different biological activities, including CRD-mediated mRNA stabilization, RNA splicing, translation, and transcription. It also has been discovered that regulation of RHA is disrupted in many types of cancers. The C. elegans rha-1 gene is expressed in the gonad where it localizes to the nucleus and the cytoplasm and is required for proper development. Defects in rha-1 lead to germline defects, aberrant expression of genes residing in repetitive transgene arrays, and defects in RNAi. ABC transporter proteins have also been implicated to play a role in RNAi in C. elegans. Ten out of the sixty-one ABC transporters are required for efficient RNAi in the germ line; these transporters are called ABCRNAi transporters. All of the ABCRNAi transporter mutants interact genetically with rde-2 (a novel protein) and mut-7 (a protein with homology to RnaseD). One of these ABCRNAi transporter genes is haf-6. The works presented here show that rha-1 mutants also interact genetically with haf-6, with rde-2, and with mut-7 with respect to RNAi defects. By contrast, mutations in rha-1 suppress the transposon mobilization phenotypes observed in haf-6 mutants. It is also observed that an unknown mutation is genetically interacting with rha-1 and haf-6. We are currently investigating roles for rha-1 in proper accumulation of haf-6 and other ABCRNAi transporter mRNAs in an effort to understand the interrelated functions of these proteins in RNAi.
    URI
    http://hdl.handle.net/1808/7747
    Collections
    • Molecular Biosciences Dissertations and Theses [270]
    • Theses [3827]

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    785-864-8983
    KU Libraries
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    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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