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dc.contributor.authorAudus, Kenneth L.
dc.contributor.authorJin, H.
dc.date.accessioned2011-06-16T16:22:47Z
dc.date.available2011-06-16T16:22:47Z
dc.date.issued2005
dc.identifier.citationJin, H. and Audus, K.L. (2005) Effect of bisphenol A on drug efflux in BeWo, a human trophoblast-like cell line. Placenta, 26 Suppl.A, S96-S103. PMID: 15837075 http://dx.doi.org/10.1016/j.placenta.2005.01.016
dc.identifier.urihttp://hdl.handle.net/1808/7611
dc.description.abstractBisphenol A (BPA) is a monomer of polycarbonate plastics that has estrogenic activities and has been shown to be a substrate for multidrug resistant efflux mechanisms, specifically, P-glycoprotein. Since the natural hormone estrogen reverses multidrug resistance in some cell types, we hypothesized that BPA might have a similar activity in trophoblasts. We have used BeWo cells as an in vitro model for human trophoblasts and calcein AM as a substrate for drug efflux mechanism to characterize BPA interactions with placental P-glycoprotein. We found that chronic exposure of BeWo cells to BPA did not alter intracellular calcein accumulation in a fashion that would be reflective of changes in P-glycoprotein expression. Immunoblots affirmed that BPA had small effects on P-glycoprotein expression. However, BeWo cells acutely exposed to BPA pretreatment were observed to have a significantly decreased calcein accumulation. Addition of cyclosporin A, a P-glycoprotein inhibitor and substrate, completely reversed BPA's effects on calcein accumulation and resulted in a net increase, relative to controls, in calcein accumulation by the BeWo cells. BPA was found not to stimulate P-gp ATPase or alter intracellular esterases mediating calcein release from calcein AM. Therefore, our results suggested that BPA stimulated drug efflux by BeWo cells probably by direct effects on P-glycoprotein.
dc.language.isoen_US
dc.publisherElsevier
dc.subjectBisphenol A (BPA)
dc.subjectP-glycoprotein (P-gp)
dc.subjectBeWo cells
dc.subjectDrug efflux
dc.subject17β-estradiol (E2)
dc.titleEffect of Bisphenol A on Drug Efflux in BeWo, a Human Trophoblast-like Cell Line
dc.typeArticle
kusw.kuauthorAudus, Kenneth L.
kusw.kudepartmentPharmacy
kusw.oastatusfullparticipation
dc.identifier.doi10.1016/j.placenta.2005.01.016
kusw.oaversionScholarly/refereed, author accepted manuscript
kusw.oapolicyThis item meets KU Open Access policy criteria.
dc.rights.accessrightsopenAccess


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