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Effect of Bisphenol A on Drug Efflux in BeWo, a Human Trophoblast-like Cell Line
dc.contributor.author | Audus, Kenneth L. | |
dc.contributor.author | Jin, H. | |
dc.date.accessioned | 2011-06-16T16:22:47Z | |
dc.date.available | 2011-06-16T16:22:47Z | |
dc.date.issued | 2005 | |
dc.identifier.citation | Jin, H. and Audus, K.L. (2005) Effect of bisphenol A on drug efflux in BeWo, a human trophoblast-like cell line. Placenta, 26 Suppl.A, S96-S103. PMID: 15837075 http://dx.doi.org/10.1016/j.placenta.2005.01.016 | |
dc.identifier.uri | http://hdl.handle.net/1808/7611 | |
dc.description.abstract | Bisphenol A (BPA) is a monomer of polycarbonate plastics that has estrogenic activities and has been shown to be a substrate for multidrug resistant efflux mechanisms, specifically, P-glycoprotein. Since the natural hormone estrogen reverses multidrug resistance in some cell types, we hypothesized that BPA might have a similar activity in trophoblasts. We have used BeWo cells as an in vitro model for human trophoblasts and calcein AM as a substrate for drug efflux mechanism to characterize BPA interactions with placental P-glycoprotein. We found that chronic exposure of BeWo cells to BPA did not alter intracellular calcein accumulation in a fashion that would be reflective of changes in P-glycoprotein expression. Immunoblots affirmed that BPA had small effects on P-glycoprotein expression. However, BeWo cells acutely exposed to BPA pretreatment were observed to have a significantly decreased calcein accumulation. Addition of cyclosporin A, a P-glycoprotein inhibitor and substrate, completely reversed BPA's effects on calcein accumulation and resulted in a net increase, relative to controls, in calcein accumulation by the BeWo cells. BPA was found not to stimulate P-gp ATPase or alter intracellular esterases mediating calcein release from calcein AM. Therefore, our results suggested that BPA stimulated drug efflux by BeWo cells probably by direct effects on P-glycoprotein. | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | |
dc.subject | Bisphenol A (BPA) | |
dc.subject | P-glycoprotein (P-gp) | |
dc.subject | BeWo cells | |
dc.subject | Drug efflux | |
dc.subject | 17β-estradiol (E2) | |
dc.title | Effect of Bisphenol A on Drug Efflux in BeWo, a Human Trophoblast-like Cell Line | |
dc.type | Article | |
kusw.kuauthor | Audus, Kenneth L. | |
kusw.kudepartment | Pharmacy | |
kusw.oastatus | fullparticipation | |
dc.identifier.doi | 10.1016/j.placenta.2005.01.016 | |
kusw.oaversion | Scholarly/refereed, author accepted manuscript | |
kusw.oapolicy | This item meets KU Open Access policy criteria. | |
dc.rights.accessrights | openAccess |
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Pharmacy Scholarly Works [299]