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dc.contributor.authorShi, Fenglin
dc.contributor.authorCavitt, Jennifer
dc.contributor.authorAudus, Kenneth L.
dc.date.accessioned2011-06-16T16:10:53Z
dc.date.available2011-06-16T16:10:53Z
dc.date.issued1995-09
dc.identifier.citationAudus, K.L., Guillot, F.L., and Braughler, J.M. (1991) Evidence for 21-aminosteroid association with the hydrophobic domains in brain microvessel endothelial cells. Free Rad. Biol. Med. 11, 361-371. PMID: 1797623 http://dx.doi.org/10.1016/0891-5849(95)00049-4
dc.identifier.urihttp://hdl.handle.net/1808/7610
dc.description.abstractSelected 21-aminosteroids (U74500A, U74006F, and U74389G) and a 2-(aminomethyl)chromans (U78517F) were tested for their efficacy in preventing arachidonate-induced lipid peroxidation and permeability alterations in brain microvessel endothelial cells (BMECs). The 21-aminosteroids and 2-(aminomethyl)chromans were effective in varying degrees in inhibiting (U74500A = U78517F> U74006F = U74389G) concentration- and time-dependent arachidonate-induced thiobarbituric acid reactive substances (TBARS) production by BMECs. Arachidonate produced a corresponding concentration-dependent increase in BMEC monolayer permeability to the membrane impermeant marker, sucrose. Pretreatment of BMEC monolayers with either the 21-aminosteroids or the 2-(aminomethyl)chromans completely blocked the arachidonate-induced increase in permeability to sucrose. Our results demonstrated that these membrane-associating antioxidants were particularly effective in preventing both arachidonic acid-induced lipid peroxidation and permeability changes in BMEC monolayers. However, concentrations of some antioxidants that only partially inhibited TBARS production, completely inhibited the arachidonic acid-induced enhancement in BMEC monolayer permeability. Therefore, arachidonic acid-induced effects on BMEC permeability were likely due in part to both lipid peroxidation and direct or indirect effects of the fatty acid on membrane integrity. This study provides further support for the application of primary cultures of BMECs as a useful in vitro system to evalulate mechanisms through which mediators of disease or injury states compromise blood-brain barrier integrity.
dc.language.isoen_US
dc.publisherElsevier
dc.subjectFree radicals
dc.subject21-Aminosteroids
dc.subject2-(Aminomethyl)chromans
dc.subjectTirilazad
dc.subjectArachidonic acid
dc.subjectBlood-brain barrier
dc.subjectBrain microvessel endothelial cells
dc.title21-aminosteroid and 2-(aminomethyl)chromans inhibition of arachidonic acid-induced lipid peroxidation and permeability enhancement in bovine brain microvessel endothelial cell monolayers
dc.typeArticle
kusw.kuauthorAudus, Kenneth L.
kusw.kudepartmentPharmacy
kusw.oastatusfullparticipation
dc.identifier.doi10.1016/0891-5849(95)00049-4
kusw.oaversionScholarly/refereed, author accepted manuscript
kusw.oapolicyThis item meets KU Open Access policy criteria.
dc.rights.accessrightsopenAccess


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