AT1 receptors mediate angiotensin II uptake and transport by brain microvessel endothelial cells in primary culture
Issue Date
1999Author
Rose, Jayna M.
Audus, Kenneth L.
Publisher
Lippincott Williams & Wilkins
Type
Article
Article Version
Scholarly/refereed, author accepted manuscript
Published Version
http://journals.lww.com/cardiovascularpharm/pages/articleviewer.aspx?year=1999&issue=01000&article=00005&type=abstractMetadata
Show full item recordAbstract
The endothelial lining of the blood-brain barrier tightly controls the distribution of peptide hormones between the central nervous system and the circulation. Using primary cultures of brain microvessel endothelial cells, an in vitro model of the blood-brain barrier, we report here the uptake and transport of the octapeptide angiotensin II by a specific receptor population. Using the angiotensin II antagonists losartan (AT1 specific) and PD 123,319 (AT2 specific) we have shown that both the uptake and transport of angiotensin II were mediated by the AT1 receptor. Western blot analysis confirmed the existence of the AT1 receptor in our cell culture model. Rhodamine 123 studies also suggested that both angiotensin II antagonists, but not angiotensin II, were substrates for the P-glycoprotein efflux system thus restricting the transport of these compounds. These results suggest an AT1 receptor mediates uptake and transport of angiotensin II at the blood-brain barrier and may contribute to the regulation of cerebrovascular levels of the peptide.
Description
This is a non-final version of an article published in final form in Rose, J.M. and Audus, K.L. (1999) AT1 receptors mediate angiotensin II uptake and transport by brain microvessel endothelial cells in primary culture. J. Cardiovasc. Pharmacol. 33, 30-35. PMID: 9890393
Collections
- Pharmacy Scholarly Works [286]
Citation
Rose, J.M. and Audus, K.L. (1999) AT1 receptors mediate angiotensin II uptake and transport by brain microvessel endothelial cells in primary culture. J. Cardiovasc. Pharmacol. 33, 30-35. PMID: 9890393
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