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    Transport and Metabolism of Opioid Peptides across BeWo Cells, An In Vitro Model of the Placental Barrier

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    Audus_AFD_Ampasavate et al Int J Pharm 2002.pdf (187.4Kb)
    Issue Date
    2002
    Author
    Ampasavate, Chadarat
    Chandorkar, Gurudatt A.
    Velde, David Vande
    Stobaugh, John F.
    Audus, Kenneth L.
    Publisher
    Elsevier
    Type
    Article
    Article Version
    Scholarly/refereed, author accepted manuscript
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    Abstract
    In keeping with the advance of biotechnology, cell culture becomes an important tool for investigating the transport and the metabolism phenomena. A cell line of human origin, the BeWo choriocarcinoma cell line, was used for the study of the transport and metabolism of opioid peptides across the in vitro model of the placental barrier. Opioid peptides, both naturally occurring and their synthetic analogs, are of interest to be developed as potent analgesics and were included in this study. The apparent permeability coefficients of the peptides containing 4 to 11 amino acid or analog residues were in the range of 0.23-14.60 x 10-5 cm/sec. The apparent permeability coefficients of these peptides were comparable to those of sucrose or dextrans, hydrophilic markers. Molecular weight and size of the peptides were inversely correlated to the permeability across the BeWo monolayers. Lipophilicity and charges of the peptides were also investigated and found to be the minor factors regulating the extent of peptide permeation. Contrasting to the transport of DPDPE peptide analog across the blood-brain barrier, the transport of DPDPE across the BeWo monolayers were not found to be via carrier-mediated transport. The major transport pathway of the opioid peptides across the BeWo monolayers was found to be via paracellular route. In metabolism studies, aminopeptidase was found to be a major enzyme type responsible for the degradation of naturally occurring peptides but not for the synthetic analogues. The finding obtained from the present study reveal the applicability of the BeWo cell line to be used as the in vitro cell culture model for the transport and metabolism screening of the opioid peptides.
    Description
    Please note that this is an author-produced PDF of an article accepted for publication following peer review. The publisher version is available on its site.
    URI
    http://hdl.handle.net/1808/7483
    DOI
    https://doi.org/10.1016/S0378-5173(01)00929-2
    Collections
    • Pharmacy Scholarly Works [286]
    Citation
    Ampasavate, C., Chandorkar, G.A, VanderVelde, D., Stobaugh, J.F., and Audus, K.L. (2002) Transport and metabolism of opioid peptides across BeWo Cells, an in vitro model of the placental barrier. Int. J. Pharm. 233, 85-98. PMID: 11897413 http://dx.doi.org/10.1016/S0378-5173(01)00929-2

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    KU Libraries
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    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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