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dc.contributor.advisorLi, Linhengen_US
dc.contributor.advisorSoares, Michaelen_US
dc.contributor.authorScoville, David
dc.date.accessioned2011-04-25T19:03:53Z
dc.date.available2011-04-25T19:03:53Z
dc.date.issued2010-06-10en_US
dc.date.submitted2010en_US
dc.identifier.otherhttp://dissertations.umi.com/ku:11023en_US
dc.identifier.urihttp://hdl.handle.net/1808/7385en_US
dc.description.abstractCritical to the identification of putative stem cell populations is the ability to evalu-ate their functional potential. Within this work I describe the development of a robust culture system which enables regeneration of the intestinal epithelium from isolated crypts and single intestinal stem cells. To discriminate putative quiescent intestinal stem cells (ISCs) from rapidly cycling Lgr5+ ISCs a tetracycline inducible H2BGFP labeling strategy was employed. Distinct populations of label and non-label retaining cells were separated via fluorescent activated cell sorting (FACS). Analysis of stem cell potential, using the intesti-nal in vitro culture system developed herein, revealed that CD166high label retaining cells (LRCs) and non-LRCs contain functional intestinal stem cells (ISCs). Gene expression analysis of these populations confirmed the expression of stem cell genes including Lgr5. Coupled with the identification of long term BrdU retaining Lgr5+ cells, this work demon-strates the existence of both active and quiescent Lgr5+ ISCs.
dc.format.extent167 pagesen_US
dc.language.isoen_USen_US
dc.publisherUniversity of Kansasen_US
dc.rightsThis item is protected by copyright and unless otherwise specified the copyright of this thesis/dissertation is held by the author.en_US
dc.subjectCell biology
dc.subjectIntestinal culture system
dc.subjectLabel retaining stem cells
dc.subjectQuiescent intestinal stem cells
dc.titleIsolation and Characterization of Quiescent Intestinal Stem Cells
dc.typeDissertationen_US
dc.contributor.cmtememberFields, Patrick E
dc.contributor.cmtememberNeufeld, Kristi
dc.contributor.cmtememberVivian, Jay L
dc.thesis.degreeDisciplinePathology & Laboratory Medicine
dc.thesis.degreeLevelPh.D.
kusw.oastatusna
kusw.oapolicyThis item does not meet KU Open Access policy criteria.
kusw.bibid8085547
dc.rights.accessrightsopenAccessen_US


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