dc.contributor.advisor | Ahmad, Syed Omar | |
dc.contributor.author | Park, Ji-Hyuk | |
dc.date.accessioned | 2010-10-03T14:06:20Z | |
dc.date.available | 2010-10-03T14:06:20Z | |
dc.date.issued | 2010-06-13 | |
dc.date.submitted | 2010 | |
dc.identifier.other | http://dissertations.umi.com/ku:10993 | |
dc.identifier.uri | http://hdl.handle.net/1808/6775 | |
dc.description.abstract | Background. 2D analyses produce systematic errors in quantifying anatomical and morphological features in the brain. Design-based stereology overcomes this limitation by applying probability theory, yet many neuroscience investigators still use 2D analyses. The purpose of this study is to compare 2D analysis with design-based stereology in quantifying differences of morphological and anatomical features between groups. Methods. Brain tissue samples of three different rodent models were analyzed; chronic MPTP/probenecid PD (MPD) mouse model, alcohol preferring (AP) rat model, and the enriched environment (EE) rat model. 2D analyses and design-based stereology were used to quantify neuronal number, neuronal volume and regional volume. Student's t-test (two-tailed) was used to compare quantitative data. Results. 2D analyses generated significantly different estimation form design-based stereology in neuronal number and did not find relatively small differences of neuronal number. 2D analysis generated comparable value to design-based stereology in normalized data but not in actual value. 2D estimated accurately regional volume. Discussion. 2D analyses may be used for rough screening to find a difference of neuronal number and volume but should not for the estimation of actual value. Design-based stereology should be used to estimate neuronal number and volume. Both 2D analyses and design based stereology can be used for the estimation of regional volume. | |
dc.format.extent | 229 pages | |
dc.language.iso | en | |
dc.publisher | University of Kansas | |
dc.rights | This item is protected by copyright and unless otherwise specified the copyright of this thesis/dissertation is held by the author. | |
dc.subject | Nanoscience | |
dc.subject | Biology | |
dc.subject | Morphology | |
dc.title | A COMPARISON OF 2D IMAGE ANALYSIS AND DESIGN-BASED STEREOLOGY FOR EVALUATING MORPHOLOGICAL AND ANATOMICAL CHANGES IN THE DOPAMINERGIC SYSTEM OF THE RODENT MIDBRAIN | |
dc.type | Dissertation | |
dc.contributor.cmtemember | Radel, Jeff | |
dc.contributor.cmtemember | Levant, Beth | |
dc.contributor.cmtemember | Stanford, John A | |
dc.contributor.cmtemember | Albertini, David F | |
dc.thesis.degreeDiscipline | Occupational Therapy Education | |
dc.thesis.degreeLevel | Ph.D. | |
kusw.oastatus | na | |
kusw.oapolicy | This item does not meet KU Open Access policy criteria. | |
kusw.bibid | 8085545 | |
dc.rights.accessrights | openAccess | |