ATTENTION: The software behind KU ScholarWorks is being upgraded to a new version. Starting July 15th, users will not be able to log in to the system, add items, nor make any changes until the new version is in place at the end of July. Searching for articles and opening files will continue to work while the system is being updated.
If you have any questions, please contact Marianne Reed at mreed@ku.edu .
A COMPARISON OF 2D IMAGE ANALYSIS AND DESIGN-BASED STEREOLOGY FOR EVALUATING MORPHOLOGICAL AND ANATOMICAL CHANGES IN THE DOPAMINERGIC SYSTEM OF THE RODENT MIDBRAIN
dc.contributor.advisor | Ahmad, Syed Omar | |
dc.contributor.author | Park, Ji-Hyuk | |
dc.date.accessioned | 2010-10-03T14:06:20Z | |
dc.date.available | 2010-10-03T14:06:20Z | |
dc.date.issued | 2010-06-13 | |
dc.date.submitted | 2010 | |
dc.identifier.other | http://dissertations.umi.com/ku:10993 | |
dc.identifier.uri | http://hdl.handle.net/1808/6775 | |
dc.description.abstract | Background. 2D analyses produce systematic errors in quantifying anatomical and morphological features in the brain. Design-based stereology overcomes this limitation by applying probability theory, yet many neuroscience investigators still use 2D analyses. The purpose of this study is to compare 2D analysis with design-based stereology in quantifying differences of morphological and anatomical features between groups. Methods. Brain tissue samples of three different rodent models were analyzed; chronic MPTP/probenecid PD (MPD) mouse model, alcohol preferring (AP) rat model, and the enriched environment (EE) rat model. 2D analyses and design-based stereology were used to quantify neuronal number, neuronal volume and regional volume. Student's t-test (two-tailed) was used to compare quantitative data. Results. 2D analyses generated significantly different estimation form design-based stereology in neuronal number and did not find relatively small differences of neuronal number. 2D analysis generated comparable value to design-based stereology in normalized data but not in actual value. 2D estimated accurately regional volume. Discussion. 2D analyses may be used for rough screening to find a difference of neuronal number and volume but should not for the estimation of actual value. Design-based stereology should be used to estimate neuronal number and volume. Both 2D analyses and design based stereology can be used for the estimation of regional volume. | |
dc.format.extent | 229 pages | |
dc.language.iso | en | |
dc.publisher | University of Kansas | |
dc.rights | This item is protected by copyright and unless otherwise specified the copyright of this thesis/dissertation is held by the author. | |
dc.subject | Nanoscience | |
dc.subject | Biology | |
dc.subject | Morphology | |
dc.title | A COMPARISON OF 2D IMAGE ANALYSIS AND DESIGN-BASED STEREOLOGY FOR EVALUATING MORPHOLOGICAL AND ANATOMICAL CHANGES IN THE DOPAMINERGIC SYSTEM OF THE RODENT MIDBRAIN | |
dc.type | Dissertation | |
dc.contributor.cmtemember | Radel, Jeff | |
dc.contributor.cmtemember | Levant, Beth | |
dc.contributor.cmtemember | Stanford, John A | |
dc.contributor.cmtemember | Albertini, David F | |
dc.thesis.degreeDiscipline | Occupational Therapy Education | |
dc.thesis.degreeLevel | Ph.D. | |
kusw.oastatus | na | |
kusw.oapolicy | This item does not meet KU Open Access policy criteria. | |
kusw.bibid | 8085545 | |
dc.rights.accessrights | openAccess |
Files in this item
This item appears in the following Collection(s)
-
Dissertations [4889]
-
Education Dissertations and Theses [1065]