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The Application of Empirical Phase Diagrams to the Biophysical Characterization and Stabilization of Viral Vaccine Candidates
Kissmann, Julian Michael
Kissmann, Julian Michael
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Abstract
This dissertation describes the application of a composite biophysical approach to the characterization and stabilization of viruses and virus-like particles for vaccine purposes. Spectroscopic, calorimetric, and light-scattering techniques were applied to detect physical changes in multiple aspects of viral architecture following the application of pharmaceutically relevant stress factors. The signals from a variety of these techniques were then combined in a vector-based approach to generate empirical phase diagrams that visually depict changes in each system's physical state as a function of experimental stress factors. These diagrams were then used to determine optimal solution storage conditions and also conditions for screening excipient libraries for appropriate stabilizers.
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Date
2010-04-20
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University of Kansas
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Keywords
Pharmaceutical chemistry, Formulation, Influenza, Measles, Stabilization, Vaccine, Virus-like particles