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dc.contributor.advisorPeltier, Edward
dc.contributor.advisorHuggins, Donald G
dc.contributor.authorEverhart, Robert Christopher
dc.date.accessioned2010-01-07T23:38:53Z
dc.date.available2010-01-07T23:38:53Z
dc.date.issued2009-12-04
dc.date.submitted2009
dc.identifier.otherhttp://dissertations.umi.com/ku:10609
dc.identifier.urihttp://hdl.handle.net/1808/5669
dc.description.abstractAntibiotics and antibiotic resistance are emerging contaminants. Tetracyclines are common antibiotics with a well known mode of action and multiple resistance determinants. Water column samples were collected from 22 streams in Kansas and Nebraska in conjunction with a USEPA probability-based study of perennial, wadeable streams. Tetracyclines were analyzed by enzyme-linked immunosorbent assay (ELISA), while polymerase chain reactions (PCR) were used to enumerate 16S-rRNA, tetW, tetQ, and tetO. TetW, tetQ, and tetO were highly correlated (r2 > 0.80, p 0.80, p tetW levels were significantly different between Kansas and Nebraska. Based on probability, approximately 20% of Kansas and Nebraska streams are predicted to have observable levels of tetracyclines, tetW, tetQ, and tetO. Findings suggest an ambient reservoir of tetracycline resistance genes and favorable conditions for resistance selection.
dc.format.extent156 pages
dc.language.isoEN
dc.publisherUniversity of Kansas
dc.rightsThis item is protected by copyright and unless otherwise specified the copyright of this thesis/dissertation is held by the author.
dc.subjectEnvironmental engineering
dc.subjectEnvironmental sciences
dc.subject16s-rrna
dc.subjectAntibiotic resistance
dc.subjectTeto
dc.subjectTetq
dc.subjectTetracycline
dc.subjectTetw
dc.titleBaseline tetracycline and tetracycline resistance levels in perennial, wadeable streams of Kansas and Nebraska
dc.typeThesis
dc.contributor.cmtememberSturm, Belinda
dc.contributor.cmtememberGraham, David W
dc.thesis.degreeDisciplineCivil, Environmental, & Architectural Engineering
dc.thesis.degreeLevelM.S.
kusw.oastatusna
kusw.oapolicyThis item does not meet KU Open Access policy criteria.
kusw.bibid7079098
dc.rights.accessrightsopenAccess


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