Show simple item record

dc.contributor.advisorBuch, Shilpa J.
dc.contributor.authorWilliams, Rachel
dc.date.accessioned2009-11-02T19:49:39Z
dc.date.available2009-11-02T19:49:39Z
dc.date.issued2009-06-23
dc.date.submitted2009
dc.identifier.otherhttp://dissertations.umi.com/ku:10414
dc.identifier.urihttp://hdl.handle.net/1808/5577
dc.description.abstractWith the prevalence of HIV-associated neurocognitive disorders (HAND) increasing, understanding the mechanisms by which HIV induces neuro-inflammation and subsequent neuronal damage is of paramount importance. We hypothesized that HIV-1 and IFN-γ/TNF-α co-operation could increase CXCL10 expression in astrocytes through redox sensitive pathways. Our initial studies focused on determining which signaling pathways were involved in CXCL10 induction in HIV-1 and cytokine treated astrocytes. The next studies were aimed at determining which HIV-1 protein was co-operating with IFN-γ and TNF-α to cause this effect. Additionally, to verify if an oxidative burst was impacting CXCL10 regulation through redox sensitive pathways we utilized apocynin, an inhibitor of NADPH oxidase. Apocynin was also able to diminish Jnk, Erk1/2, and Akt pathway activation,decrease NF-κB activation and decrease CXCL10 expression, improving neuronal survival. This data has implications for the development of therapeutic strategies aimed at reducing the release of pro-inflammatory agents to prevent HIV-1 neuropathogenesis.
dc.format.extent146 pages
dc.language.isoEN
dc.publisherUniversity of Kansas
dc.rightsThis item is protected by copyright and unless otherwise specified the copyright of this thesis/dissertation is held by the author.
dc.subjectBiology
dc.subjectNeurosciences
dc.subjectMolecular biology
dc.subjectMicrobiology
dc.subjectAstrocytes
dc.subjectCxcl10
dc.subjectHiv
dc.subjectHiv-associated dementia
dc.subjectIfn-gamma
dc.subjectTnf-alpha
dc.titleHIV-Encephalitis: Mechanisms for CXCL10 Induction in Astrocytes
dc.typeDissertation
dc.contributor.cmtememberGonzalez, Norberto C.
dc.contributor.cmtememberKim, Kee Jun
dc.contributor.cmtememberNewell, Kathy
dc.contributor.cmtememberYankee, Thomas
dc.thesis.degreeDisciplineMolecular & Integrative Physiology
dc.thesis.degreeLevelPh.D.
kusw.oastatusna
kusw.oapolicyThis item does not meet KU Open Access policy criteria.
kusw.bibid7078943
dc.rights.accessrightsopenAccess


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record