EFFECT OF DIETARY GLYCOMACROPEPTIDE AND CHOLESTEROL ON CORTICAL GANGLIOSIDE- AND GLYCOPROTEIN-BOUND N-ACETYLNEURAMINIC ACID IN YOUNG RATS

View/ Open
Issue Date
2009-04-29Author
Kary, Susan Ann
Publisher
University of Kansas
Format
88 pages
Type
Thesis
Degree Level
M.S.
Discipline
Dietetics & Nutrition
Rights
This item is protected by copyright and unless otherwise specified the copyright of this thesis/dissertation is held by the author.
Metadata
Show full item recordAbstract
Background: Sialic acid and cholesterol are present in human milk and accumulate
rapidly in the brain during development. Infant formulas contain little sialic acid or
cholesterol. Sialic acid and cholesterol supplementation increase cortical ganglioside
and glycoprotein sialic acid and cholesterol in animal models, respectively. Doseresponse
studies and those varying both sialic acid and cholesterol intake are lacking.
Objective: The purpose of this study was to determine the effects of bovine casein
glycomacropeptide (CGMP) and dietary cholesterol on cortical ganglioside- and
glycoprotein-bound sialic acid accumulation in young rats.
Design/Methods: Dams were fed a nutritionally complete rat diet with 0 or 0.5%
cholesterol by weight throughout pregnancy and lactation. Litters were culled to 8
pups on postnatal day (P) 1. Coagulated milk was removed from the stomach of P1
pups and the cholesterol concentration determined. After weaning, two pups from
each litter were allocated to one of four diets varying in CGMP (provided
approximately 0, 20, 40 and 80 mg sialic acid/kg d-1) and the same cholesterol
concentration as their dams. In the first experiment, the dams’ litters were weaned on
P17 to the sialic acid-supplemented diets and sacrificed on P32. In the second
experiment, litters were weaned on P21 to the diet of their dams. Pups consumed the
dams’ diets until P24 or P33 and their assigned sialic acid-supplemented diets
thereafter. The pups were sacrificed between P38 and P47, 14 days after the initiation
of the sialic acid-supplemented diets. On the day of sacrifice, pups were decapitated
and their brains immediately frozen on dry ice. Gangliosides and glycoproteins were extracted from the cortex and the sialic acid concentration determined.
Results: Maternal cholesterol intake increased total milk cholesterol (p = 0.0009). In
pups sacrificed on P32, CGMP increased cortical ganglioside sialic acid in a doseresponse
manner (p for trend = 0.007). In the same experiment, dietary cholesterol
independently increased ganglioside sialic acid (p = 0.02). In pups sacrificed between
P38 and P47, cholesterol intake increased glycoprotein sialic acid (p = 0.030).
Conclusions: Increased sialic acid and cholesterol consumption during the first 4
weeks of postnatal brain growth influenced brain composition in rats.
Collections
- Education Dissertations and Theses [1065]
- Theses [3906]
Items in KU ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
We want to hear from you! Please share your stories about how Open Access to this item benefits YOU.