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    REGULATORY MECHANISMS OF SLC39A4 (ZIP4) AND SLC39A5 (ZIP5) IN THE ADAPTIVE RESPONSE TO ZINC AVAILABILITY

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    Weaver_ku_0099D_10235_DATA_1.pdf (6.207Mb)
    Issue Date
    2009-04-06
    Author
    Weaver, Benjamin Patrick
    Publisher
    University of Kansas
    Format
    283 pages
    Type
    Dissertation
    Degree Level
    Ph.D.
    Discipline
    Biochemistry & Molecular Biology
    Rights
    This item is protected by copyright and unless otherwise specified the copyright of this thesis/dissertation is held by the author.
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    Abstract
    The aims of this research were to determine how Zip4 and Zip5 are regulated in response to zinc availability and how Zip4 impacts development. Loss of Zip4 resulted in embryonic lethality. Heterozygosity negatively affected eye, heart, and brain development. Excess zinc did not rescue lethality but ameliorated the heterozygous effects. Zip4 and Zip5 had reciprocal regulation in response to zinc availability. Zip4 expression was regulated by stability of the mRNA and protein: both accumulate during zinc deficiency; ZIP4 was rapidly internalized then degraded following zinc repletion. The Zip5 mRNA levels did not change with zinc availability and were polysome-associated. ZIP5 accumulated on the basolateral membranes after zinc repletion. miRNAs predicted to target Zip5 in an accessible region of the conserved 3' UTR were polysome-associated in all tissues that regulate Zip5. Zip4 and Zip5 are both regulated by post-transcriptional mechanisms in response to zinc availability and Zip4 is essential for development.
    URI
    http://hdl.handle.net/1808/5391
    Collections
    • Dissertations [4473]
    • Molecular Biosciences Dissertations and Theses [273]

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    785-864-8983

    KU Libraries
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    Lawrence, KS 66045
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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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