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    Tissue-Targeted Metabonomics: Metabolic Profiling by Microdialysis and NMR Spectroscopy

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    Price_ku_0099D_10065_DATA_1.pdf (3.347Mb)
    Issue Date
    2008-01-01
    Author
    Price, Kristin Elizabeth
    Publisher
    University of Kansas
    Format
    257 pages
    Type
    Dissertation
    Degree Level
    Ph.D.
    Discipline
    Chemistry
    Rights
    This item is protected by copyright and unless otherwise specified the copyright of this thesis/dissertation is held by the author.
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    Abstract
    This research offers an alternative approach to the study of tissue-specific metabolism by metabonomics, called tissue-targeted metabonomics. Microdialysis sampling was used to sample small endogenous metabolites in tissue extracellular fluid. Samples were then analyzed by proton nuclear magnetic resonance spectroscopy. The research explored analytical and biological considerations for this method. Data treatment, including integration of the NMR spectra and normalization to standards, was optimized for different dialysate types. Time trends in the basal metabolism of liver and heart tissues were described, as were the metabolic effects of anesthesia, circadian rhythms and animal activity. After the method was developed and basal metabolism was adequately characterized, tissue-targeted metabonomics was used to study oxidative stress, induced locally by perfusing doxorubicin through a probe in the liver. Results indicated a localized metabolic response to the doxorubicin dose in awake rats, illustrating the potential of tissue-targeted metabonomics to monitor localized metabolic changes for many applications.
    URI
    http://hdl.handle.net/1808/4513
    Collections
    • Dissertations [4473]
    • Chemistry Dissertations and Theses [336]

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    785-864-8983
    KU Libraries
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    785-864-8983

    KU Libraries
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    Lawrence, KS 66045
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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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