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    Tissue Engineering the TMJ Condyle using Human Umbilical Cord Mesenchymal Stromal Cells

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    Issue Date
    2008-09-09
    Author
    Wang, Limin
    Publisher
    University of Kansas
    Format
    260 pages
    Type
    Dissertation
    Degree Level
    Ed.D.
    Discipline
    Chemical & Petroleum Engineering
    Rights
    This item is protected by copyright and unless otherwise specified the copyright of this thesis/dissertation is held by the author.
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    Abstract
    The temporomandibular joint (TMJ), associated with everyday activities, such as chewing, yawning, talking, and laughing, is one of the most complex but least studied joints in the musculoskeletal system. Patients suffering from TMJ disorders (TMDs) may experience a variety of agonizing symptoms, such as earaches, headaches, neck pain, and difficulty opening the mouth, while current treatments are inefficacious owing to a poor understanding of TMJ disorder pathologies. Prior to this thesis, human umbilical cord mesenchymal stromal cells (hUCMSCs), identified as multipotent cells only in this decade, had not yet been used for TMJ tissue engineering. Hence, this thesis proposed a revolutionary tissue engineering approach in which hUCMSCs and mature hyaline cartilage cells, scaffolds, and growth factors were integrated to create TMJ condylar bone and cartilage in vitro to substitute for deteriorated native tissues. hUCMSCs were successfully differentiated along chondrogenic and osteogenic lineages in a 3D biomaterial-based environment, supporting the feasibility of using these cells for TMJ cartilage and bone tissue engineering. In TMJ cartilage regeneration, hUCMSCs demonstrated significant advantages over both mature TMJ cells and human bone marrow mesenchymal stromal cells (hBMSCs), with faster cell proliferation and superior biosynthesis. As an additional alternative, hyaline cartilage cells also surpassed TMJ cells in that they produced considerably more extracellular matrix. Following the initial efforts, the cell culture environment was refined, including cell seeding densities and signaling strategies. Higher cell seeding densities (25 million cells/ml) were recommended for both cartilage and bone tissue engineering, mainly due to their benefits to differentiation and biosynthesis. Insulin-like growth factor I (IGF-I) enhanced the chondrogenesis of pre-differentiated hUCMSCs while having no effect on osteogenically induced hUCMSCs. Therefore, the work on the successful differentiation of hUCMSCs in 3D biomaterials has been pioneering, and the culture parameters for in vitro TMJ tissue engineering have been refined. Moreover, this innovative work has tremendous implications for a broader area (e.g., musculoskeletal tissue engineering).
    URI
    http://hdl.handle.net/1808/4316
    Collections
    • Engineering Dissertations and Theses [1055]
    • Dissertations [4321]

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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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