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    Structure and Dynamic Studies of the Nuclear Pore Complex at the Single-Molecule Level

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    umi-ku-2634_1.pdf (5.438Mb)
    Issue Date
    2008-08-06
    Author
    Dickenson, Nicholas E.
    Publisher
    University of Kansas
    Format
    236 pages
    Type
    Dissertation
    Degree Level
    PH.D.
    Discipline
    Chemistry
    Rights
    This item is protected by copyright and unless otherwise specified the copyright of this thesis/dissertation is held by the author.
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    Abstract
    Nuclear pore complexes (NPCs) are large macromolecular structures forming the only known direct route across the double bilayer membrane of the nuclear envelope. The NPC structure has been extensively explored in an effort to elucidate the mechanisms by which they control transport. Many of these studies have found the presence of a central mass or plug within the central channel of NPCs, although neither the function nor identity of the central mass were clear. Here, several techniques including electron microscopy, Förster resonance energy transfer (FRET), and high-resolution near-field scanning optical microscopy (NSOM) are utilized to specifically locate vault ribonucleoproteins to NPCs. This interaction, along with several other results, strongly suggests that vaults represent the central mass of NPCs. A single-molecule transport assay was also developed in order to record the translocation of individual fluorescent dextrans through NPCs. Comparison of the single-molecule dwell times under various conditions led to a better understanding of the specific mechanism controlling the non signal-mediated transport of cargo through NPCs.
    URI
    http://hdl.handle.net/1808/4181
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    • Chemistry Dissertations and Theses [336]
    • Dissertations [4475]

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    785-864-8983
    KU Libraries
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    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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