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N6-methyladenosine modification of a parvovirus-encoded small noncoding RNA facilitates viral DNA replication through recruiting Y-family DNA polymerases
dc.contributor.author | Ning, Kang | |
dc.contributor.author | Zhao, Junxing | |
dc.contributor.author | Feng, Zehua | |
dc.contributor.author | Park, Soo Yeun | |
dc.contributor.author | McFarlin, Shane | |
dc.contributor.author | Cheng, Fang | |
dc.contributor.author | Yan, Ziying | |
dc.contributor.author | Wang, Jingxin | |
dc.contributor.author | Qiu, Jianming | |
dc.date.accessioned | 2024-07-08T20:22:09Z | |
dc.date.available | 2024-07-08T20:22:09Z | |
dc.date.issued | 2024-06-14 | |
dc.identifier.citation | Ning K, Zhao J, Feng Z, Park SY, McFarlin S, Cheng F, Yan Z, Wang J, Qiu J. N6-methyladenosine modification of a parvovirus-encoded small noncoding RNA facilitates viral DNA replication through recruiting Y-family DNA polymerases. Proc Natl Acad Sci U S A. 2024 Jun 18;121(25):e2320782121. doi: 10.1073/pnas.2320782121. Epub 2024 Jun 14. PMID: 38875150; PMCID: PMC11194592 | en_US |
dc.identifier.uri | https://hdl.handle.net/1808/35442 | |
dc.description.abstract | Human bocavirus 1 (HBoV1) is a human parvovirus that causes lower respiratory tract infections in young children. It contains a single-stranded (ss) DNA genome of ~5.5 kb that encodes a small noncoding RNA of 140 nucleotides known as bocavirus-encoded small RNA (BocaSR), in addition to viral proteins. Here, we determined the secondary structure of BocaSR in vivo by using DMS-MaPseq. Our findings reveal that BocaSR undergoes N6-methyladenosine (m6A) modification at multiple sites, which is critical for viral DNA replication in both dividing HEK293 cells and nondividing cells of the human airway epithelium. Mechanistically, we found that m6A-modified BocaSR serves as a mediator for recruiting Y-family DNA repair DNA polymerase (Pol) η and Pol κ likely through a direct interaction between BocaSR and the viral DNA replication origin at the right terminus of the viral genome. Thus, this report represents direct involvement of a viral small noncoding RNA in viral DNA replication through m6A modification. | en_US |
dc.publisher | PNAS | en_US |
dc.rights | This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND). | en_US |
dc.rights.uri | Copyright © 2024 the Author(s). Published by PNAS. | en_US |
dc.subject | M6A modification | en_US |
dc.subject | Viral noncoding RNA | en_US |
dc.subject | Parvovirus | en_US |
dc.subject | DNA replication | en_US |
dc.title | N6-methyladenosine modification of a parvovirus-encoded small noncoding RNA facilitates viral DNA replication through recruiting Y-family DNA polymerases | en_US |
dc.type | Article | en_US |
kusw.kuauthor | Zhao, Junxing | |
kusw.kudepartment | Department of Microbiology, Molecular Genetics and Immunology | en_US |
dc.identifier.doi | 10.1073/pnas.2320782121 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0003-1654-9770 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0002-6305-6218 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0001-6881-9168 | en_US |
dc.identifier.orcid | https://orcid.org/0009-0002-1817-7915 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0001-9850-1695 | en_US |
kusw.oaversion | Scholarly/refereed, publisher version | en_US |
kusw.oapolicy | This item meets KU Open Access policy criteria. | en_US |
dc.rights.accessrights | openAccess | en_US |
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