Show simple item record

dc.contributor.advisorBuechner, Matthew
dc.contributor.authorYang, Zhe
dc.date.accessioned2023-07-04T18:17:09Z
dc.date.available2023-07-04T18:17:09Z
dc.date.issued2020-05-31
dc.date.submitted2020
dc.identifier.otherhttp://dissertations.umi.com/ku:17231
dc.identifier.urihttps://hdl.handle.net/1808/34529
dc.description.abstractSingle-celled tubules represent one of the most difficult structures to form during development, requiring extension of a narrow cytoplasm surrounding a lumen exerting osmotic pressure that can burst the luminal membrane. Genetic studies on the excretory canal cell of C. elegans have revealed many proteins that regulate the cytoskeleton, vesicular transport, and physiology of the narrow canals. Here, we show that βH-spectrin SMA-1 regulates the placement of intermediate filament proteins IFA-4 and EXC-2/IFC-2 to form a terminal web around the lumen, and that the terminal web EXC-2 in turn retains a highly conserved protein (EXC-9/CRIP1) that regulates apical endosomal trafficking. EXC-1/IRG, the binding partner of EXC-9, is also localized to the apical membrane and affects apical actin placement and RAB-8-mediated vesicular transport. The results suggest that an intermediate filament protein acts in a novel signal transduction pathway to direct the traffic of vesicular cargo to locations of lengthening apical surface during single-celled tubule development.
dc.format.extent110 pages
dc.language.isoen
dc.publisherUniversity of Kansas
dc.rightsCopyright held by the author.
dc.subjectGenetics
dc.subjectCellular biology
dc.subjectDevelopmental biology
dc.subjectC. elegans
dc.subjectCytoskeleton
dc.subjectEndosome
dc.subjectIntermediate filament
dc.subjectTubulogenesis
dc.titleTerminal Web and Associated Proteins Mediate Signaling to Promote Nematode Single-Cell Tubulogenesis
dc.typeDissertation
dc.contributor.cmtememberXu, Liang
dc.contributor.cmtememberTimmons, Lisa
dc.contributor.cmtememberDentler, William
dc.contributor.cmtememberWard, Robert
dc.contributor.cmtememberGleason, Jennifer
dc.contributor.cmtememberBuechner, Matthew
dc.thesis.degreeDisciplineMolecular Biosciences
dc.thesis.degreeLevelPh.D.
dc.identifier.orcidhttps://orcid.org/0000-0003-3232-0626en_US
dc.rights.accessrightsopenAccess


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record