dc.contributor.author | Eades, Jason | |
dc.contributor.author | Audiffred, Julianne F. | |
dc.contributor.author | Fincher, Micah | |
dc.contributor.author | Choi, Jin-Woo | |
dc.contributor.author | Soper, Steven A. | |
dc.contributor.author | Monroe, William Todd | |
dc.date.accessioned | 2023-05-09T18:34:08Z | |
dc.date.available | 2023-05-09T18:34:08Z | |
dc.date.issued | 2023-01-22 | |
dc.identifier.citation | Eades, J.; Audiffred, J.F.; Fincher, M.; Choi, J.-W.; Soper, S.A.; Monroe, W.T. A Simple Micromilled Microfluidic Impedance Cytometer with Vertical Parallel Electrodes for Cell Viability Analysis. Micromachines 2023, 14, 283. https://doi.org/10.3390/mi14020283 | en_US |
dc.identifier.uri | https://hdl.handle.net/1808/34147 | |
dc.description.abstract | Microfluidic impedance cytometry has been demonstrated as an effective platform for single cell analysis, taking advantage of microfabricated features and dielectric cell sensing methods. In this study, we present a simple microfluidic device to improve the sensitivity, accuracy, and throughput of single suspension cell viability analysis using vertical sidewall electrodes fabricated by a widely accessible negative manufacturing method. A microchannel milled through a 75 µm platinum wire, which was embedded into poly-methyl-methacrylate (PMMA), created a pair of parallel vertical sidewall platinum electrodes. Jurkat cells were interrogated in a custom low-conductivity buffer (1.2 ± 0.04 mS/cm) to reduce current leakage and increase device sensitivity. Confirmed by live/dead staining and electron microscopy, a single optimum excitation frequency of 2 MHz was identified at which live and dead cells were discriminated based on the disruption in the cell membrane associated with cell death. At this frequency, live cells were found to exhibit changes in the impedance phase with no appreciable change in magnitude, while dead cells displayed the opposite behavior. Correlated with video microscopy, a computational algorithm was created that could identify cell detection events and determine cell viability status by application of a mathematical correlation method. | en_US |
dc.publisher | MDPI | en_US |
dc.rights | © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license. | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en_US |
dc.subject | Single cell | en_US |
dc.subject | Microfluidic impedance cytometry | en_US |
dc.subject | Impedance spectroscopy | en_US |
dc.subject | Micromilling | en_US |
dc.subject | Vertical electrodes | en_US |
dc.subject | Sidewall electrodes | en_US |
dc.title | A Simple Micromilled Microfluidic Impedance Cytometer with Vertical Parallel Electrodes for Cell Viability Analysis | en_US |
dc.type | Article | en_US |
kusw.kuauthor | Soper, Steven A. | |
kusw.kudepartment | Center of Biomodular Multiscale Systems for Precision Medicine | en_US |
kusw.kudepartment | Chemistry | en_US |
dc.identifier.doi | 10.3390/mi14020283 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0001-6364-1604 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0002-9210-9681 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0002-8292-7058 | en_US |
dc.identifier.orcid | https://orcid.org/0000-0002-7889-3799 | en_US |
kusw.oaversion | Scholarly/refereed, publisher version | en_US |
kusw.oapolicy | This item meets KU Open Access policy criteria. | en_US |
dc.identifier.pmid | PMC9959585 | en_US |
dc.rights.accessrights | openAccess | en_US |