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    Measurement of Adenovirus-Based Vector Heterogeneity (Dataset)

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    Hickey_JPharmSci_Data.zip (9.444Mb)
    Issue Date
    2022
    Author
    Hickey, John M.
    Jacob, Shaleem I.
    Tait, Andrew S.
    Dastjerdi, Fatemeh V.
    Barritt, Joseph
    Rouse, Sarah
    Douglas, Alexander
    Joshi, Sangeeta B.
    Volkin, David B.
    Bracewell, Daniel G.
    Publisher
    Elsevier
    Type
    Dataset
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    Abstract
    Adenovirus vectors have become an important class of vaccines with the recent approval of Ebola and COVID-19 products. In-process quality attribute data collected during Adenovirus vector manufacturing has focused on particle concentration and infectivity ratios (based on viral genome: cell-based infectivity), and data suggest only a fraction of viral particles present in the final vaccine product are efficacious. To better understand this product heterogeneity, lab-scale preparations of two Adenovirus viral vectors, (Chimpanzee adenovirus (ChAdOx1) and Human adenovirus Type 5 (Ad5), were studied using transmission electron microscopy (TEM). Different adenovirus morphologies were characterized, and the proportion of empty and full viral particles were quantified. Interestingly, these proportions correlated with the sample’s infectivity values. Liquid chromatography-mass spectrometry (LC-MS) peptide mapping was used to identify and track key adenovirus proteins involved in viral maturation. Using peptide abundance analysis, a ~5-fold change in L1 52/55k abundance was observed between low-(empty) and high-density (full) fractions taken from CsCl ultracentrifugation preparations of ChAdOx1 virus. The L1 52/55k viral protein is associated with DNA packaging and is cleaved during viral maturation, so it may be a marker for infective particles. TEM and LC-MS peptide mapping are thus promising higher-resolution analytical characterization tools to help differentiate between relative proportions of empty, non-infectious, and infectious viral particles as part of Adenovirus vector in-process monitoring.
    URI
    http://hdl.handle.net/1808/33700
    DOI
    https://doi.org/10.17161/1808.33700
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    • Pharmaceutical Chemistry Scholarly Works [336]

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    KU Libraries
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    1425 Jayhawk Blvd
    Lawrence, KS 66045
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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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