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dc.contributor.authorTembe, Vrushali
dc.descriptionM.S. University of Kansas, Chemistry 1998en_US
dc.description.abstractIn the recent past there has been a controversy about the basal glucose levels in the brain of freely moving rats. Kinetic rate constants and transport parameters across the blood brain barrier and in the brain tissue obtained by using different techniques support a glucose value of 2-4mM. One paper, using microdialysis in awake freely moving animals has reported a value of 0.47 mM which is several fold lower than the currently accepted value.

Microdialysis was used to determine the basal concentration of glucose in the striatum of freely moving rats. While there are several approaches to calibrating brain microdialysis probes that subsequently allow determination of the tissue concentration, the zero net flux method at a fixed perfusion flow rate, was used to make such determinations. The basal glucose concentrations were found to vary with variation in perfusion flow rate. The in vivo recoveries did not equal in vivo deliveries. To probe glucose dynamics in the brain, pharmacological agents such as glucose inhibitors and sodium channel blockers were used. In addition, to determine concentration profiles with the microdialysis probes inserted in tissue, a mathematical model was developed which took into account transport and metabolism of glucose.

In conclusion, the measured basal values of glucose depend on the perfusion flow rate when a zero net flux calibration is performed. The dialysate glucose levels are closely related to neuronal activity. When non linear kinetics dominate in the tissue, in vivo recoveries and deliveries are not equal, at least for endogenous glucose. Increases in in vivo delivery at higher flow rates could be due to factors such as saturable transport of glucose across the blood brain barrier and its subsequent uptake by neuronal cells.
dc.publisherUniversity of Kansasen_US
dc.rightsThis item is protected by copyright and unless otherwise specified the copyright of this thesis/dissertation is held by the author.en_US
dc.titleMethodological considerations of microdialysis sampling for determination of cerebral basal glucose concentration in extracellular space of freely moving raten_US
kusw.oanotes2022/11/29: Added to KUSW with the permission of the author: From: Vrushali Tembe <> Date: November 27, 2022 at 5:42:13 PM CST To: "Wilson, George S." <> Subject: Re: FW: M.S. Thesis

Dear Dr. Wilson,

What a great idea! Yes, I grant my thesis to be deposited into KUworks.

And such pleasure to hear from you. Hope all is well with you!

Although not an excuse, my work at Edwards Lifesciences and my family keep me busy, please accept my sincere apologies for not replying back sooner. I currently work in Regulatory Affairs, and the company is known for pioneering transcatheter heart valves. We have 2 daughters, a junior at UC Berkeley, pursuing BioEngineering and a senior in high school, interested in business/entrepreneurship. Yoli and I meet often and talk about

Let me know if you need anything else, I'd be happy to help!

Best regards, Vrushali (mobile): 510-396-8515

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