Functional Profiling of Antibody Immune Repertoires in Convalescent Zika Virus Disease Patients
dc.contributor.author | Fahad, Ahmed S. | |
dc.contributor.author | Timm, Morgan R. | |
dc.contributor.author | Madan, Bharat | |
dc.contributor.author | Burgomaster, Katherine E. | |
dc.contributor.author | Dowd, Kimberly A. | |
dc.contributor.author | Normandin, Erica | |
dc.contributor.author | Gutiérrez-González, Matías F. | |
dc.contributor.author | Pennington, Joseph M. | |
dc.contributor.author | De Souza, Matheus Oliveira | |
dc.contributor.author | Henry, Amy R. | |
dc.contributor.author | Laboune, Farida | |
dc.contributor.author | Wang, Lingshu | |
dc.contributor.author | Ambrozak, David R. | |
dc.contributor.author | Gordon, Ingelise J. | |
dc.contributor.author | Douek, Daniel C. | |
dc.contributor.author | Ledgerwood, Julie E. | |
dc.contributor.author | Graham, Barney S. | |
dc.contributor.author | Castilho, Leda R. | |
dc.contributor.author | Pierson, Theodore C. | |
dc.contributor.author | Mascola, John R. | |
dc.contributor.author | DeKosky, Brandon J. | |
dc.date.accessioned | 2022-01-07T19:18:41Z | |
dc.date.available | 2022-01-07T19:18:41Z | |
dc.date.issued | 2021-02-24 | |
dc.identifier.citation | Fahad, A. S., Timm, M. R., Madan, B., Burgomaster, K. E., Dowd, K. A., Normandin, E., Gutiérrez-González, M. F., Pennington, J. M., De Souza, M. O., Henry, A. R., Laboune, F., Wang, L., Ambrozak, D. R., Gordon, I. J., Douek, D. C., Ledgerwood, J. E., Graham, B. S., Castilho, L. R., Pierson, T. C., Mascola, J. R., … DeKosky, B. J. (2021). Functional Profiling of Antibody Immune Repertoires in Convalescent Zika Virus Disease Patients. Frontiers in immunology, 12, 615102. https://doi.org/10.3389/fimmu.2021.615102 | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/32364 | |
dc.description.abstract | The re-emergence of Zika virus (ZIKV) caused widespread infections that were linked to Guillain-Barré syndrome in adults and congenital malformation in fetuses, and epidemiological data suggest that ZIKV infection can induce protective antibody responses. A more detailed understanding of anti-ZIKV antibody responses may lead to enhanced antibody discovery and improved vaccine designs against ZIKV and related flaviviruses. Here, we applied recently-invented library-scale antibody screening technologies to determine comprehensive functional molecular and genetic profiles of naturally elicited human anti-ZIKV antibodies in three convalescent individuals. We leveraged natively paired antibody yeast display and NGS to predict antibody cross-reactivities and coarse-grain antibody affinities, to perform in-depth immune profiling of IgM, IgG, and IgA antibody repertoires in peripheral blood, and to reveal virus maturation state-dependent antibody interactions. Repertoire-scale comparison of ZIKV VLP-specific and non-specific antibodies in the same individuals also showed that mean antibody somatic hypermutation levels were substantially influenced by donor-intrinsic characteristics. These data provide insights into antiviral antibody responses to ZIKV disease and outline systems-level strategies to track human antibody immune responses to emergent viral infections. | en_US |
dc.publisher | Frontiers Media | en_US |
dc.rights | Copyright © 2021 Fahad, Timm, Madan, Burgomaster, Dowd, Normandin, Gutierrez-Gonza ́ lez, Pennington, De Souza, Henry, Laboune, Wang, Ambrozak, ́ Gordon, Douek, Ledgerwood, Graham, Castilho, Pierson, Mascola and DeKosky. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en_US |
dc.subject | Zika virus (ZIKV) | en_US |
dc.subject | Antiviral antibodies | en_US |
dc.subject | B cells | en_US |
dc.subject | Yeast display | en_US |
dc.subject | Next-generation sequencing | en_US |
dc.title | Functional Profiling of Antibody Immune Repertoires in Convalescent Zika Virus Disease Patients | en_US |
dc.type | Article | en_US |
kusw.kuauthor | Fahad, Ahmed S. | |
kusw.kuauthor | Madan, Bharat | |
kusw.kuauthor | Gutiérrez-González, Matías F. | |
kusw.kuauthor | Pennington, Joseph M. | |
kusw.kuauthor | De Souza, Matheus Oliveira | |
kusw.kuauthor | DeKosky, Brandon J. | |
kusw.kudepartment | Pharmaceutical Chemistry | en_US |
kusw.kudepartment | Chemical Engineering | en_US |
dc.identifier.doi | 10.3389/fimmu.2021.615102 | en_US |
kusw.oaversion | Scholarly/refereed, publisher version | en_US |
kusw.oapolicy | This item meets KU Open Access policy criteria. | en_US |
dc.identifier.pmid | PMC7959826 | en_US |
dc.rights.accessrights | openAccess | en_US |
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Except where otherwise noted, this item's license is described as: Copyright © 2021 Fahad, Timm, Madan, Burgomaster, Dowd, Normandin, Gutierrez-Gonza ́ lez, Pennington, De Souza, Henry, Laboune, Wang, Ambrozak, ́ Gordon, Douek, Ledgerwood, Graham, Castilho, Pierson, Mascola and DeKosky. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).