Show simple item record

dc.contributor.authorFahad, Ahmed S.
dc.contributor.authorTimm, Morgan R.
dc.contributor.authorMadan, Bharat
dc.contributor.authorBurgomaster, Katherine E.
dc.contributor.authorDowd, Kimberly A.
dc.contributor.authorNormandin, Erica
dc.contributor.authorGutiérrez-González, Matías F.
dc.contributor.authorPennington, Joseph M.
dc.contributor.authorDe Souza, Matheus Oliveira
dc.contributor.authorHenry, Amy R.
dc.contributor.authorLaboune, Farida
dc.contributor.authorWang, Lingshu
dc.contributor.authorAmbrozak, David R.
dc.contributor.authorGordon, Ingelise J.
dc.contributor.authorDouek, Daniel C.
dc.contributor.authorLedgerwood, Julie E.
dc.contributor.authorGraham, Barney S.
dc.contributor.authorCastilho, Leda R.
dc.contributor.authorPierson, Theodore C.
dc.contributor.authorMascola, John R.
dc.contributor.authorDeKosky, Brandon J.
dc.date.accessioned2022-01-07T19:18:41Z
dc.date.available2022-01-07T19:18:41Z
dc.date.issued2021-02-24
dc.identifier.citationFahad, A. S., Timm, M. R., Madan, B., Burgomaster, K. E., Dowd, K. A., Normandin, E., Gutiérrez-González, M. F., Pennington, J. M., De Souza, M. O., Henry, A. R., Laboune, F., Wang, L., Ambrozak, D. R., Gordon, I. J., Douek, D. C., Ledgerwood, J. E., Graham, B. S., Castilho, L. R., Pierson, T. C., Mascola, J. R., … DeKosky, B. J. (2021). Functional Profiling of Antibody Immune Repertoires in Convalescent Zika Virus Disease Patients. Frontiers in immunology, 12, 615102. https://doi.org/10.3389/fimmu.2021.615102en_US
dc.identifier.urihttp://hdl.handle.net/1808/32364
dc.description.abstractThe re-emergence of Zika virus (ZIKV) caused widespread infections that were linked to Guillain-Barré syndrome in adults and congenital malformation in fetuses, and epidemiological data suggest that ZIKV infection can induce protective antibody responses. A more detailed understanding of anti-ZIKV antibody responses may lead to enhanced antibody discovery and improved vaccine designs against ZIKV and related flaviviruses. Here, we applied recently-invented library-scale antibody screening technologies to determine comprehensive functional molecular and genetic profiles of naturally elicited human anti-ZIKV antibodies in three convalescent individuals. We leveraged natively paired antibody yeast display and NGS to predict antibody cross-reactivities and coarse-grain antibody affinities, to perform in-depth immune profiling of IgM, IgG, and IgA antibody repertoires in peripheral blood, and to reveal virus maturation state-dependent antibody interactions. Repertoire-scale comparison of ZIKV VLP-specific and non-specific antibodies in the same individuals also showed that mean antibody somatic hypermutation levels were substantially influenced by donor-intrinsic characteristics. These data provide insights into antiviral antibody responses to ZIKV disease and outline systems-level strategies to track human antibody immune responses to emergent viral infections.en_US
dc.publisherFrontiers Mediaen_US
dc.rightsCopyright © 2021 Fahad, Timm, Madan, Burgomaster, Dowd, Normandin, Gutierrez-Gonza ́ lez, Pennington, De Souza, Henry, Laboune, Wang, Ambrozak, ́ Gordon, Douek, Ledgerwood, Graham, Castilho, Pierson, Mascola and DeKosky. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).en_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.subjectZika virus (ZIKV)en_US
dc.subjectAntiviral antibodiesen_US
dc.subjectB cellsen_US
dc.subjectYeast displayen_US
dc.subjectNext-generation sequencingen_US
dc.titleFunctional Profiling of Antibody Immune Repertoires in Convalescent Zika Virus Disease Patientsen_US
dc.typeArticleen_US
kusw.kuauthorFahad, Ahmed S.
kusw.kuauthorMadan, Bharat
kusw.kuauthorGutiérrez-González, Matías F.
kusw.kuauthorPennington, Joseph M.
kusw.kuauthorDe Souza, Matheus Oliveira
kusw.kuauthorDeKosky, Brandon J.
kusw.kudepartmentPharmaceutical Chemistryen_US
kusw.kudepartmentChemical Engineeringen_US
dc.identifier.doi10.3389/fimmu.2021.615102en_US
kusw.oaversionScholarly/refereed, publisher versionen_US
kusw.oapolicyThis item meets KU Open Access policy criteria.en_US
dc.identifier.pmidPMC7959826en_US
dc.rights.accessrightsopenAccessen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

Copyright © 2021 Fahad, Timm, Madan, Burgomaster, Dowd, Normandin, Gutierrez-Gonza ́ lez, Pennington, De Souza, Henry, Laboune, Wang, Ambrozak, ́ Gordon, Douek, Ledgerwood, Graham, Castilho, Pierson, Mascola and DeKosky. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).
Except where otherwise noted, this item's license is described as: Copyright © 2021 Fahad, Timm, Madan, Burgomaster, Dowd, Normandin, Gutierrez-Gonza ́ lez, Pennington, De Souza, Henry, Laboune, Wang, Ambrozak, ́ Gordon, Douek, Ledgerwood, Graham, Castilho, Pierson, Mascola and DeKosky. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).