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    Staphylococcus aureus and Fatty Acids: Impact on Membrane Function and Signaling

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    Available after: 2021-12-31 (3.836Mb)
    Issue Date
    2021-05-31
    Author
    DeMars, Zachary
    Publisher
    University of Kansas
    Format
    210 pages
    Type
    Dissertation
    Degree Level
    Ph.D.
    Discipline
    Microbiology, Molecular Genetics & Immunology
    Rights
    Copyright held by the author.
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    Abstract
    Staphylococcus aureus can utilize exogenous fatty acids for phospholipid synthesis. The fatty acid kinase FakA is essential for this utilization by phosphorylating exogenous fatty acids for incorporation into lipids. How exogenous fatty acid utilization from the environment via FakA affects the composition and function of the phospholipid membrane, central metabolism, and virulence factor regulation is not well understood. We found that inactivation of the exogenous fatty acid utilization system through genetic deletion of fakA resulted in a membrane that is more rigid than membranes of wild-type S. aureus. Central metabolism, particularly the production and consumption of acetate, was significantly altered resulting in a more oxidized cellular environment when fakA was deleted. Both the accumulation of free fatty acids within the cell of a fakA mutant and the presence of exogenous fatty acids in the growth medium decrease virulence factor production through the transmembrane domains of the two-component system SaeRS. In summary, we identified previously unknown roles for the exogenous fatty acid utilization system in maintaining membrane and metabolic homeostasis in addition to expanding our mechanistic understanding of how fatty acids negatively influence virulence factor expression.
    URI
    http://hdl.handle.net/1808/31767
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    • Dissertations [4473]

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    785-864-8983
    KU Libraries
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    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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