Show simple item record

dc.contributor.authorPhillips, Kelly N.
dc.contributor.authorWidmann, Scott
dc.contributor.authorLai, Huei-Yi
dc.contributor.authorNguyen, Jennifer
dc.contributor.authorRay, J. Christian J.
dc.contributor.authorBalázsi, Gábor
dc.contributor.authorCooper, Tim F.
dc.identifier.citationPhillips KN, Widmann S, Lai H-Y, Nguyen J, Ray JCJ, Balázsi G, Cooper TF. 2019. Diversity in lac operon regulation among diverse Escherichia coli isolates depends on the broader genetic background but is not explained by genetic relatedness. mBio 10:e02232-19.
dc.descriptionThis work is licensed under a Creative Commons Attribution 4.0 International License.en_US
dc.description.abstractTranscription of bacterial genes is controlled by the coordinated action of cis- and trans-acting regulators. The activity and mode of action of these regulators can reflect different requirements for gene products in different environments. A well-studied example is the regulatory function that integrates the environmental availability of glucose and lactose to control the Escherichia coli lac operon. Most studies of lac operon regulation have focused on a few closely related strains. To determine the range of natural variation in lac regulatory function, we introduced a reporter construct into 23 diverse E. coli strains and measured expression with combinations of inducer concentrations. We found a wide range of regulatory functions. Several functions were similar to the one observed in a reference lab strain, whereas others depended weakly on the presence of cAMP. Some characteristics of the regulatory function were explained by the genetic relatedness of strains, indicating that differences varied on relatively short time scales. The regulatory characteristics explained by genetic relatedness were among those that best predicted the initial growth of strains following transition to a lactose environment, suggesting a role for selection. Finally, we transferred the lac operon, with the lacI regulatory gene, from five natural isolate strains into a reference lab strain. The regulatory function of these hybrid strains revealed the effect of local and global regulatory elements in controlling expression. Together, this work demonstrates that regulatory functions can be varied within a species and that there is variation within a species to best match a function to particular environments.en_US
dc.publisherAmerican Society for Microbiologyen_US
dc.rightsCopyright © 2019 Phillips et al.en_US
dc.subjectLac operon regulationen_US
dc.titleDiversity in lac Operon Regulation among Diverse Escherichia coli Isolates Depends on the Broader Genetic Background but Is Not Explained by Genetic Relatednessen_US
kusw.kuauthorRay, J. Christian J.
kusw.kudepartmentCenter for Computational Biologyen_US
kusw.kudepartmentMolecular Biosciencesen_US
kusw.oaversionScholarly/refereed, publisher versionen_US
kusw.oapolicyThis item meets KU Open Access policy criteria.en_US

Files in this item


This item appears in the following Collection(s)

Show simple item record

Copyright © 2019 Phillips et al.
Except where otherwise noted, this item's license is described as: Copyright © 2019 Phillips et al.