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dc.contributor.authorMcCann, Clare M.
dc.contributor.authorChristgen, Beate
dc.contributor.authorRoberts, Jennifer A.
dc.contributor.authorSu, Jian-Qiang
dc.contributor.authorArnold, Kathryn E.
dc.contributor.authorGray, Neil D.
dc.contributor.authorZhu, Yong-Guan
dc.contributor.authorGraham, David W.
dc.date.accessioned2021-01-07T21:55:55Z
dc.date.available2021-01-07T21:55:55Z
dc.date.issued2019-01-28
dc.identifier.citationClare M. McCann, Beate Christgen, Jennifer A. Roberts, Jian-Qiang Su, Kathryn E. Arnold, Neil D. Gray, Yong-Guan Zhu, David W. Graham, "Understanding drivers of antibiotic resistance genes in High Arctic soil ecosystems", Environment International, Volume 125, 2019, Pages 497-504, ISSN 0160-4120, https://doi.org/10.1016/j.envint.2019.01.034.en_US
dc.identifier.urihttp://hdl.handle.net/1808/31064
dc.descriptionThis work is licensed under a Creative Commons Attribution 4.0 International License.en_US
dc.description.abstractSoils in tropical and temperate locations are known to be a sink for the genetic potential of anthropogenic-driven acquired antibiotic resistance (AR). In contrast, accumulation of acquired AR is less probable in most Polar soils, providing a platform for characterizing background resistance and establishing a benchmark for assessing AR spread. Here, high-throughput qPCR and geochemistry were used to quantify the abundance and diversity of both antibiotic resistance genes (ARGs) and selected mobile genetic elements (MGEs) across eight soil clusters in the Kongsfjorden region of Svalbard in the High Arctic. Relative ARG levels ranged by over two orders of magnitude (10−6 to 10−4 copies/16S rRNA gene copy), and showed a gradient of potential human and wildlife impacts across clusters as evidenced by altered geochemical conditions and increased “foreign” ARG abundances (i.e., allochthonous), including blaNDM-1. Impacted clusters exhibited 100× higher total ARGs and MGEs in tandem with elevated secondary nutrients, especially available P that is typically low and limiting in Arctic soils. In contrast, ARGs in less-impacted clusters correlated strongly to local soil lithology. The most plausible source of exogenous P and allochthonous ARGs in this region is bird and other wildlife guano, disseminated either by local human wastes or via direct carriage and deposition. Regardless of pathway, accumulation of apparent allochthonous ARGs and MGEs in High Arctic soils is concerning, highlighting the importance of characterizing Arctic sites now to establish benchmarks for tracking AR spread around the world.en_US
dc.publisherElsevieren_US
dc.rights© 2019 The Authors. Published by Elsevier Ltd.en_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.subjectHigh Arcticen_US
dc.subjectAntibiotic resistanceen_US
dc.subjectInternational spreaden_US
dc.subjectWildlifeen_US
dc.subjectGeochemistryen_US
dc.subjectHigh throughput qPCRen_US
dc.titleUnderstanding drivers of antibiotic resistance genes in High Arctic soil ecosystemsen_US
dc.typeArticleen_US
kusw.kuauthorRoberts, Jennifer A.
kusw.kudepartmentGeologyen_US
dc.identifier.doi10.1016/j.envint.2019.01.034en_US
kusw.oaversionScholarly/refereed, publisher versionen_US
kusw.oapolicyThis item meets KU Open Access policy criteria.en_US
dc.rights.accessrightsopenAccessen_US


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© 2019 The Authors. Published by Elsevier Ltd.
Except where otherwise noted, this item's license is described as: © 2019 The Authors. Published by Elsevier Ltd.