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dc.contributor.authorColicchio, Jack M.
dc.contributor.authorKelly, John K.
dc.contributor.authorHileman, Lena C.
dc.identifier.citationColicchio, J. M., Kelly, J. K., & Hileman, L. C. (2018). Parental experience modifies the Mimulus methylome. BMC genomics, 19(1), 746.
dc.descriptionThis work is licensed under a Creative Commons Attribution 4.0 International License.en_US
dc.description.abstractBackground Transgenerational plasticity occurs when the environmental experience of an organism modifies the growth and development of its progeny. Leaf damage in Mimulus guttatus exhibits transgenerational plasticity mediated through differential expression of hundreds of genes. The epigenetic mechanisms that facilitate this response have yet to be described.

Results We performed whole genome bisulfite sequencing in the progeny of genetically identical damaged and control plants and developed a pipeline to compare differences in the mean and variance of methylation between treatment groups. We find that parental damage increases the variability of CG and CHG methylation among progeny, but does not alter the overall mean methylation. Instead it has positive effects in some regions and negative in others. We find 3,396 CHH, 203 CG, and 54 CHG Differentially Methylated Regions (DMRs) ranging from tens to thousands of base pairs scattered across the genome. CHG and CHH DMRs tended to overlap with transposable elements. CG DMRs tended to overlap with gene coding regions, many of which were previously found to be differentially expressed.

Conclusions Genome-wide increases in methylome variation suggest that parental conditions can increase epigenetic diversity in response to stress. Additionally, the potential association between CG DMRs and differentially expressed genes supports the hypothesis that differential methylation is a mechanistic component of transgenerational plasticity in M. guttatus.
dc.description.sponsorshipUniversity of Kansas Botany Endowmenten_US
dc.description.sponsorshipNSF IOS-0951254en_US
dc.description.sponsorshipKansas-INBRE P20-GM103418en_US
dc.rights© The Author(s). 2018en_US
dc.subjectMimulus guttatusen_US
dc.subjectTransgenerational plasticityen_US
dc.titleParental experience modifies the Mimulus methylomeen_US
kusw.kuauthorColicchio, Jack M.
kusw.kuauthorKelly, John K.
kusw.kuauthorHileman, Lena C.
kusw.kudepartmentEcology and Evolutionary Biologyen_US
kusw.oanotesPer Sherpa Romeo 12/01/2020:

BMC Genomics [Open panel below]Publication Information TitleBMC Genomics [English] ISSNsElectronic: 1471-2164 URL PublishersBMC [Commercial Publisher] DOAJ Listing Requires APCYes [Data provided by DOAJ] [Open panel below]Publisher Policy Open Access pathways permitted by this journal's policy are listed below by article version. Click on a pathway for a more detailed view.

Published Version NoneCC BYPMC Any Website, Journal Website, +3 OA PublishingThis pathway includes Open Access publishing EmbargoNo Embargo LicenceCC BY Copyright OwnerAuthors Publisher Deposit PubMed Central Europe PubMed Central Location Any Website Author's Homepage Institutional Repository Named Repository (PubMed Central) Journal Website Conditions Copy of License must accompany any deposit. Published source must be acknowledged Must link to publisher version with DOI
kusw.oaversionScholarly/refereed, publisher versionen_US
kusw.oapolicyThis item meets KU Open Access policy criteria.en_US

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© The Author(s). 2018
Except where otherwise noted, this item's license is described as: © The Author(s). 2018