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dc.contributor.authorSubramanian, Jaichandar
dc.contributor.authorMichel, Katrin
dc.contributor.authorBenoit, Marc
dc.contributor.authorNedivi, Elly
dc.date.accessioned2019-11-07T22:07:28Z
dc.date.available2019-11-07T22:07:28Z
dc.date.issued2019-08-06
dc.identifier.citationSubramanian, J., Michel, K., Benoit, M., & Nedivi, E. (2019). CPG15/Neuritin Mimics Experience in Selecting Excitatory Synapses for Stabilization by Facilitating PSD95 Recruitment. Cell reports, 28(6), 1584–1595.e5. doi:10.1016/j.celrep.2019.07.012en_US
dc.identifier.urihttp://hdl.handle.net/1808/29730
dc.description.abstractA key feature of brain plasticity is the experience-dependent selection of optimal connections, implemented by a set of activity-regulated genes that dynamically adjust synapse strength and number. The activity-regulated gene cpg15/neuritin has been previously implicated in stabilization and maturation of excitatory synapses. Here, we combine two-photon microscopy with genetic and sensory manipulations to dissect excitatory synapse formation in vivo and examine the role of activity and CPG15 in dendritic spine formation, PSD95 recruitment, and synapse stabilization. We find that neither visual experience nor CPG15 is required for spine formation. However, PSD95 recruitment to nascent spines and their subsequent stabilization requires both. Further, cell-autonomous CPG15 expression is sufficient to replace experience in facilitating PSD95 recruitment and spine stabilization. CPG15 directly interacts with α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors on immature dendritic spines, suggesting a signaling mode for this small extracellular molecule acting as an experience-dependent “selector” for spine stabilization and synapse maturation.en_US
dc.publisherElsevieren_US
dc.rights© 2019 The Authors This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International Licenseen_US
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/en_US
dc.subjectActivity-regulated genesen_US
dc.subjectDendritic spinesen_US
dc.subjectExcitatory synapsesen_US
dc.subjectVisual experienceen_US
dc.subjectCPG15en_US
dc.subjectNeuritinen_US
dc.subjectPSD95en_US
dc.subjectAMPA receptorsen_US
dc.subjectIn vivo two-photon microscopyen_US
dc.titleCPG15/Neuritin Mimics Experience in Selecting Excitatory Synapses for Stabilization by Facilitating PSD95 Recruitmenten_US
dc.typeArticleen_US
kusw.kuauthorSubramanian, Jaichandar
kusw.kudepartmentPharmacology and Toxicologyen_US
dc.identifier.doi10.1016/j.celrep.2019.07.012en_US
kusw.oaversionScholarly/refereed, publisher versionen_US
kusw.oapolicyThis item meets KU Open Access policy criteria.en_US
dc.rights.accessrightsopenAccessen_US


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© 2019 The Authors This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
Except where otherwise noted, this item's license is described as: © 2019 The Authors This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License