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dc.contributor.authorJack, Brittany
dc.contributor.authorMueller, David M.
dc.contributor.authorFee, Ann C.
dc.contributor.authorTetlow, Ashley L.
dc.contributor.authorAvasthi, Prachee
dc.date.accessioned2019-09-06T14:37:34Z
dc.date.available2019-09-06T14:37:34Z
dc.date.issued2019-05-21
dc.identifier.citationBrittany Jack, David M. Mueller, Ann C. Fee, Ashley L. Tetlow, Prachee Avasthi, Partially Redundant Actin Genes in Chlamydomonas Control Transition Zone Organization and Flagellum-Directed Traffic, Cell Reports, Volume 27, Issue 8, 2019, Pages 2459-2467.e3, ISSN 2211-1247, https://doi.org/10.1016/j.celrep.2019.04.087.en_US
dc.identifier.urihttp://hdl.handle.net/1808/29541
dc.descriptionA grant from the One-University Open Access Fund at the University of Kansas was used to defray the author's publication fees in this Open Access journal. The Open Access Fund, administered by librarians from the KU, KU Law, and KUMC libraries, is made possible by contributions from the offices of KU Provost, KU Vice Chancellor for Research & Graduate Studies, and KUMC Vice Chancellor for Research. For more information about the Open Access Fund, please see http://library.kumc.edu/authors-fund.xml.en_US
dc.description.abstractThe unicellular green alga Chlamydomonas reinhardtii is a biflagellated cell with two actin genes: one encoding a conventional actin (IDA5) and the other encoding a divergent novel actin-like protein (NAP1). Here, we probe how actin redundancy contributes to flagellar assembly. Disrupting a single actin allows complete flagellar assembly. However, when disrupting both actins using latrunculin B (LatB) treatment on the nap1 mutant background, we find that actins are necessary for flagellar growth from newly synthesized limiting flagellar proteins. Under total actin disruption, transmission electron microscopy identified an accumulation of Golgi-adjacent vesicles. We also find that there is a mislocalization of a key transition zone gating and ciliopathy protein, NPHP-4. Our experiments demonstrate that each stage of flagellar biogenesis requires redundant actin function to varying degrees, with an absolute requirement for these actins in transport of Golgi-adjacent vesicles and flagellar incorporation of newly synthesized proteins.en_US
dc.description.sponsorshipP20 GM104936-09en_US
dc.description.sponsorship1R35GM128702-01en_US
dc.description.sponsorshipNSF GRFP grant 1518767en_US
dc.publisherElsevieren_US
dc.relation.ispartofseriesCell Reports;27
dc.relation.isversionofhttps://www.sciencedirect.com/science/article/pii/S2211124719305674?via%3Dihub#!en_US
dc.rightsThis article is available under the terms of the Creative Commons Attribution License (CC BY). You may copy and distribute the article, create extracts, abstracts and new works from the article, alter and revise the article, text or data mine the article and otherwise reuse the article commercially (including reuse and/or resale of the article) without permission from Elsevier. You must give appropriate credit to the original work, together with a link to the formal publication through the relevant DOI and a link to the Creative Commons user license above. You must indicate if any changes are made but not in any way that suggests the licensor endorses you or your use of the work.

Permission is not required for this type of reuse.
en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.subjectChlamydomonas reinhardtiien_US
dc.subjectciliaen_US
dc.subjectactinen_US
dc.subjecttraffickingen_US
dc.subjectflagellar assemblyen_US
dc.subjectGoldi-adjacent vesiclesen_US
dc.subjecttransition zone compositionen_US
dc.subjectflagellar protein synthesisen_US
dc.titlePartially Redundant Actin Genes in Chlamydomonas Control Transition Zone Organization and Flagellum-Directed Trafficen_US
dc.typeArticleen_US
kusw.kuauthorAvasthi, Prachee
kusw.kuauthorMueller, David M.
kusw.kuauthorJack, Brittany
kusw.kudepartmentAnatomy and Cell Biologyen_US
kusw.kudepartmentOphtamology/ KUMCen_US
dc.identifier.doi10.1016/j.celrep.2019.04.087en_US
dc.identifier.orcidhttps://orcid.org/0000-0002-1688-722Xen_US
kusw.oaversionScholarly/refereed, publisher versionen_US
kusw.oapolicyThis item meets KU Open Access policy criteria.en_US
dc.rights.accessrightsopenAccessen_US


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You may copy and distribute the article, create extracts, abstracts and new works from the article, alter and revise the article, text or data mine the article and otherwise reuse the article commercially (including reuse and/or resale of the article) without permission from Elsevier. You must give appropriate credit to the original work, together with a link to the formal publication through the relevant DOI and a link to the Creative Commons user license above. You must indicate if any changes are made but not in any way that suggests the licensor endorses you or your use of the work.

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Except where otherwise noted, this item's license is described as: This article is available under the terms of the Creative Commons Attribution License (CC BY). You may copy and distribute the article, create extracts, abstracts and new works from the article, alter and revise the article, text or data mine the article and otherwise reuse the article commercially (including reuse and/or resale of the article) without permission from Elsevier. You must give appropriate credit to the original work, together with a link to the formal publication through the relevant DOI and a link to the Creative Commons user license above. You must indicate if any changes are made but not in any way that suggests the licensor endorses you or your use of the work. Permission is not required for this type of reuse.