KUKU

KU ScholarWorks

  • myKU
  • Email
  • Enroll & Pay
  • KU Directory
    • Login
    View Item 
    •   KU ScholarWorks
    • Dissertations and Theses
    • Dissertations
    • View Item
    •   KU ScholarWorks
    • Dissertations and Theses
    • Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Measurements of neurotransmitter release in animal models of the central nervous system using fast scan cyclic voltammetry

    Thumbnail
    View/Open
    Available after: 2019-12-31 (3.372Mb)
    Issue Date
    2017-12-31
    Author
    GINTHER, RACHEL C.
    Publisher
    University of Kansas
    Format
    139 pages
    Type
    Dissertation
    Degree Level
    Ph.D.
    Discipline
    Chemistry
    Rights
    Copyright held by the author.
    Metadata
    Show full item record
    Abstract
    This dissertation is a compilation of the work we have done in the past five years using fast-scan cyclic voltammetry in model organisms to solve biological problems. In this work, we use fast-scan cyclic voltammetry to measure neurotransmitter release in rodent models of neurodegeneration and neurotoxicity. Later, we develop this technique for use in zebrafish whole mount retinas. In Chapter One, we will introduce fast-scan cyclic voltammetry and the underlying electrochemical concepts. We will also introduce the various biological problems investigated in this work and the model organisms used to investigate these problems. In Chapter Two, we will discuss fast-scan cyclic voltammetry measurements of serotonin release in Huntington’s disease model mice. We have demonstrated that serotonin release is impaired in multiple regions of the brain and across multiple mouse models. In Chapter Three, we will discuss fast-scan cyclic voltammetric measurements of both dopamine and serotonin in chemotherapy-treated rats. Here, we have treated rats with both carboplatin and 5-fluorouracil in order to investigate the effect of chemotherapy on neurotransmitter release. Additionally, we will discuss our collaboration with Dr. David Jarmolowicz, in which his lab measured cognitive behavioral changes in rats treated with chemotherapy. We have also investigated the novel drug, KU-32, developed by Dr. Brian Blagg’s group, as a potential therapy for chemotherapy-induced cognitive changes. In Chapter Four, we will discuss our work developing a method to measure light-stimulated neurotransmitter release in adult zebrafish whole mount retinas. Here, we have shown through pharmacological studies that dopamine can be measured using fast-scan cyclic voltammetry in the retina. Finally, in Chapter Five, we will present our conclusions and future directions for this work.
    URI
    http://hdl.handle.net/1808/27839
    Collections
    • Chemistry Dissertations and Theses [335]
    • Dissertations [4454]

    Items in KU ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.


    We want to hear from you! Please share your stories about how Open Access to this item benefits YOU.


    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

    Browse

    All of KU ScholarWorksCommunities & CollectionsThis Collection

    My Account

    Login

    Statistics

    View Usage Statistics

    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

    The University of Kansas
      Contact KU ScholarWorks
    Lawrence, KS | Maps
     
    • Academics
    • Admission
    • Alumni
    • Athletics
    • Campuses
    • Giving
    • Jobs

    The University of Kansas prohibits discrimination on the basis of race, color, ethnicity, religion, sex, national origin, age, ancestry, disability, status as a veteran, sexual orientation, marital status, parental status, gender identity, gender expression and genetic information in the University’s programs and activities. The following person has been designated to handle inquiries regarding the non-discrimination policies: Director of the Office of Institutional Opportunity and Access, IOA@ku.edu, 1246 W. Campus Road, Room 153A, Lawrence, KS, 66045, (785)864-6414, 711 TTY.

     Contact KU
    Lawrence, KS | Maps